Now showing items 21-30 of 32
Mutagen-induced diploid human lymphoblast variants containing altered hypoxanthine guanine phosphoribosyl transferase
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1977-03)
The human lymphoblast line MGL8 was treated with HAT and subsequently “mutagenized” with EMS (200 μg/ml) to give 15% survival, and 6-thioguanine-resistant cells were selected by cloning in soft agarose containing the drug ...
Modification of Drosophila cell surfaces by concanavalin A
(Springer-Verlag, 1977-12)
The effect of Con A on the surface morphology of cultured cells of Drosophilia melanogaster growing on coverglasses was examined by scanning electron microscopy. With low lectin concentrations (5–10μg/ml) surface filaments ...
Species and tissue differences in the catabolism of S-adenosyl-L-homocysteine: A quantitative, chromatographic study
(Elsevier, 1977-01-15)
Using a rapid, quantitative high pressure liquid chromatographic (HPLC) procedure for the separation of S-adenosyl-L-homocysteine (AdoHcy) and its purinic metabolites in rat and mouse brain, kidney and liver, we found that ...
Argininosuccinate synthetase activity in cultured human lymphocytes
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1977-04)
The activity of argininosuccinate synthetase (E.C. 6.3.4.5), a urea cycle enzyme, was measured in cultured human lymphocytes using a new radioactive assay. Control cells had a maximum specific activity of 15.7±8.7 nmoles ...
Stimulation of protein synthesis "in vitro" by transcripts of mouse myeloma chromatin
(Elsevier, 1977-07-11)
SummaryWheat germ RNA polymerase II has been used to synthesize RNA's from mouse myeloma chromatin. The newly synthesized RNA has been shown to stimulate amino acid incorporation in a cell-free protein synthesizing system ...
Enzyme regulation during development and aging
(Elsevier, 1977-04-25)
Glutamine synthetase and ornithine aminotransferase activities have been shown to decrease in aging rats, but the decline varies in degree for the two enzymes in liver and kidney. The responsiveness of the two liver enzymes ...
Regulation of testicular LH receptors by homologous hormone: studies on receptor occupancy and receptor loss
(Elsevier, 1977-02-21)
A method is described which makes use of 4M MgCl2 to dissociate the testicular luteinizing hormone-receptor complex without altering either the binding capacity or binding affinity of the receptor. Using this method, it ...
Genetic control of quantitative variation in carbonic anhydrase isozymes of mammals. I. The concentration within single erythrocytes of the mouse
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1977-10)
The levels of the carbonic anhydrase isozymes (CA and CA II) in single erythrocytes of DBA/2J mice were assayed by measuring the specific immunofluorescence of CA I and CA II with a microspectrofluorometric technique. ...
Respiratory water loss: A predictive model
(Elsevier, 1977-03-21)
A steady-state model, based on a combination of empirical and mechanistic relationships, is developed to predict respiratory water loss from terrestrial vertebrates. Model parameters are evaluated from published data for ...
The subcellular distribution of acyl CoA: Dihydroxyacetone phosphate acyl transferase in guinea pig liver
(Elsevier, 1977-06-20)
Upon differential centrifugation, the enzyme acyl CoA: dihydroxyacetone phosphate acyl transferase (EC 2.3.1.42) in guinea pig liver is shown to sediment in a lysosomal-peroxisomal fraction. Comparison of the distribution ...