Now showing items 11-20 of 85
A solid support for affinity chromatography that covalently binds thiol groups via a cleavable connector arm
(Elsevier, 1980-09)
Preparation of an agarose derivative (MPE-agarose) containing a maleimido group which is attached to agarose via a cleavable phenyl ester linkage is described. MPE-agarose was shown to react with the thiol groups in ...
Mechanisms of gentamicin-induced dysfunction of renal cortical mitochondria : II. Effects on mitochondrial monovalent cation transport
(Elsevier, 1980-11)
Mitochondrial swelling techniques were used to evaluate the effects of the aminoglycoside antibiotic gentamicin on renal cortical mitochondrial monovalent cation permeability. Gentamicin behaved like EDTA to enhance ...
Comparison of the phospholipid requirements and molecular form of CTP: Phosphocholine cytidylyltransferase from rat lung, kidney, brain and liver
(Elsevier, 1980-12-05)
The Cytidylyltransferase activity in fresh cytosol from different tissues of the rat was measured in the absence and presence of phosphatidylglycerol. In all cases addition of this lipid produced large increases in enzyme ...
NMR relaxivity changes in chloroplast suspensions. Effects of NH2OH and of treatments altering the redox state of the photosynthetic electron transport chain
(Elsevier, 1980-12-03)
Treatments (illumination, chemical oxidation or reduction) which are potentially capable of producing paramagnetic centers in chloroplast thylakoid membranes do not produce enhancements of the proton magnetic relaxivities ...
Protection of cyclooxygenase activity during heme-induced destabilization
(Elsevier, 1980-05)
Sheep vesicular gland cyclooxygenase is destroyed spontaneously when incubated with only substoichiometric amounts of heme. Peroxides may participate in this destruction, since glutathione peroxidase, catalase, and phenol, ...
Rapid purification of yeast mitochondrial DNA in high yield
(Elsevier, 1980-12-11)
A procedure is presented for the rapid isolation of mitochondrial DNA (mtDNA) in high yield from Saccharomyces cerevisiae. Yeast cells, which may be grown to late stationary phase, are broken by a combination of enzymatic ...
A comparison of cholesteryl oleate and 19-iodocholesteryl oleate as substrates for adrenal cholesterol esterase
(Elsevier, 1980-04)
Radiolabeled 19-iodocholesterol is widely used to obtain images of human adrenals. We have shown in rats and mice that 85-95% of the radiolabel is present in the esterified form. Using rat adrenal cytosolic cholesterol ...
Spectrophotometric determination of chromium as the chromium-peroxo-4-(2-pyridylazo)resorcinol complex
(Springer-Verlag, 1980-01)
Der ternäre Chrom-Peroxo-PAR-Komplex weist einen scheinbaren molaren Extinktionskoeffizienten von 6280 l · mol −1 · cm −1 auf, wenn er aus 0,1 M schwefelsaurer Lösung mit Ethylacetat extrahiert wird. Das Beersche Gesetz ...
Aromatic antimony compounds. Transition metal complexes of 2,5-dimethylstibacyclopentadienyl
(Elsevier, 1980-12-23)
Hydrostannation of 2,4-hexadiyne with dibutyltin dihydride gave 1,1-dibutyl-2,5-dimethylstannole, which was converted to 2,5-dimethylstibacymantrene and to bis(2,5-dimethylstibacyclopentadienyl)iron.
Reduced lateral mobility of a fluorescent lipid probe in cholesterol-depleted erythrocyte membrane
(Elsevier, 1980-03-27)
The effect of cholesterol depletion of the human erythrocyte membrane on the lateral diffusion rate of a fluorescent lipid probe is reported. At low temperatures (-5 to 5[deg]C), the diffusion of the probe is 50% slower ...