Now showing items 71-80 of 81
Reverse-phase high-performance liquid chromatography of hydrophobic proteins and fragments thereof
(Elsevier, 1983-05)
Reverse-phase high-performance liquid chromatography (HPLC) resolution and recovery of cytochrome P-450 and bovine rhodopsin, both integral membrane proteins, and large peptides derived from P-450 LM2 were enhanced by ...
The cytokinetic and cytotoxic effects of ICRF-159 and ICRF-187 in vitro and ICRF-187 in human bone marrow in vivo
(Kluwer Academic Publishers; Martinus Nijhoff Publishers ; Springer Science+Business Media, 1983-12)
The cytotoxic and cytokinetic effects of ICRF-159 and its d-enantiomer ICRF-187 have been examined in vitro . The effects of both agents were identical. Cytotoxicity is dependent on both the drug concentration and the ...
Dimerization occurs during the reversible acid inactivation of 2-oxo-4-hydroxyglutarate aldolase from Escherichia coli
(Elsevier, 1983-11-28)
Exposure of Escherichia coli 2-oxo-4-hydroxyglutarate aldolase (4-hydroxy-2-oxoglutarate glyoxylate-lyase, EC 4.1.3.16) (molecular WEIGHT = 63000) to phosphoric acid at pH 1.6 for 10 min at 4[delta]C causes 95% or greater ...
Fate of intravenously administered high-density lipoprotein labeled with radioiodinated cholesteryl oleate in normal and hypolipidemic rats
(Elsevier, 1983-03-01)
Radioiodinated cholesteryl oleate (125I-CO) was found to associate rapidly with plasma lipoproteins following intravenous administration to rats. The high-density lipoprotein (HDL) fraction was observed to contain the ...
Two-dimensional peptide mapping by reversed-phase column chromatography, applied to the sequence determination of cytochrome c from the wild type and a mutant of the butterfly, pieris brassicae
(Elsevier, 1983-08-26)
Two-dimensional peptide mapping has been very effective in the characterization of protein digests, particularly for the detection of small structural differences between homologous proteins. The classical thin-layer ...
Sandstone water sensitivity: Existence of a critical rate of salinity decrease for particle capture
(Elsevier, 1983)
A critical rate of salinity decrease (CRSD) was found to exist in the water sensitivity of Berea sandstone. The CRSD was determined by conducting core flood experiments in which the salinity was decreased exponentially by ...
Hydrogen aspiration in a direct injection type diesel engine-its effects on smoke and other engine performance parameters
(Elsevier, 1983)
An experimental study was undertaken to investigate the possibility of reducing diesel particulates in the exhaust by aspirating small quantities of gaseous hydrogen in the intake of a diesel engine. A single cylinder, ...
An assay for the rates of cleavage of specific sites in DNA by restriction endonucleases: Its use to study the cleavage of phage [lambda] DNA by EcoRI and phage P22 DNA containing thymine or 5-bromouracil by HindIII
(Elsevier, 1983-03)
A method to measure the rates of cleavage of specific sites in DNAs by restriction endonucleases is described. Partial digests are prepared by incubating DNAs with limiting amounts of endonuclease. The termini generated ...
Measurement of nucleoside kinases in crude tissue extracts
(Elsevier, 1983-08)
The measurements of deoxyadenosine kinase, adenosine kinase, and deoxycytidine kinase were examined in human placental cytosol to achieve a valid and reliable assay linear with time and protein. Our studies confirm the ...
Is cytochrome aa3 from thermus thermophilus a single subunit oxidase?
(Elsevier, 1983)
A reliable procedure has been developed for the purification of the cytochrome c1aa3 complex from the plasma membrane of T. thermophilus. The ratios heme C:heme A:Fe:C were found to be 1:2:3:2 confirming previous results, ...