Now showing items 51-60 of 93
In situ hybridization histochemistry with synthetic oligonucleotides: Strategies and methods
(Elsevier, 1985)
In situ hybridization histochemistry is a useful method for localizing specific mRNA and studying the regulation of gene expression in an anatomical context. Previously, classical recombinant DNA and microbiological ...
Optical probe study of a nonentangling macromolecule solution--bovine serum albumin: Water
(Elsevier, 1985-06)
The diffusion coefficient D of polystyrene latex spheres in bovine serum albumin:water was studied as a function of protein concentration c for 0 c D fails by as much as 25 to 50%, D being larger than predicted from the ...
Analysis of plasma cyanate as 2-nitro-5-thiocarbamylbenzoic acid by high-performance liquid chromatography
(Elsevier, 1985-05-01)
A method for the determination of cyanate concentration in blood plasma over the range 1 to 1000 [mu] is presented. Cyanate present in the dried residue of acetone-deproteinized plasma is converted to a chromophoric ...
Quarter field resonance and integer-spin/half-spin interaction in the EPR of Thermus thermophilus ferrodoxin. Possible new fingerprints for three iron clusters
(Elsevier, 1985-04-29)
We describe two new characteristics of the EPR of the seven-iron containing ferrodoxin from Thermus thermophilus. First, the reduced state of the 3Fe center, which has traditionally been considered to be EPR-silent, has ...
Stereospecific synthesis of [gamma]-butyrolactones from acyclic vinyl sulfoxides: an asymmetric synthesis of optically pure oak lactones
(Elsevier, 1985)
The sulfoxide-directed lactonization of several trisubstituted vinyl sulfoxides with dichloroketene proceeds in a completely stereospecific manner. The lactonization of (E) -3 (R) --tolylsulfinyloctene occurs with complete ...
Function of ribosomes and glutamyl-tRNA isoacceptors in protein synthesis in regenerating skeletal muscle
(Elsevier, 1985-04-19)
Ribosomes from 8-day-regenerating rat skeletal muscle have been shown to be more active in poly(U)-directed polyphenylalanine synthesis than ribosomes from control muscle. This difference persists after salt washing of the ...
Fischer-Tropsch synthesis on bimetallic ruthenium-gold catalysts
(Elsevier, 1985-06)
The effect of Au in the behavior of Ru as a Fischer-Tropsch catalyst was studied. Two series of catalysts were investigated, one supported on SiO2 and the other on MgO. Au did not seem to alter the product distribution on ...
The measurement of testosterone and oestradiol-17[beta] using iodinated tracers and incorporating an affinity chromatography extraction procedure
(Elsevier, 1985-12)
The development of sensitive radioimmunoassays (RIA) for testosterone and oestradiol-17[beta], utilising 125I-radioligands, is described. Use of an homologous bridge at the same site of attachment, for both the radioligand ...
Protein synthesis by hepatic mitochondria isolated from carbon tetrachloride-exposed rats
(Elsevier, 1985-03-20)
Hepatic mitochondria isolated from rats 40 h after dosage with 1.1 ml/kg CCl4 are uncoupled and display structural damage. Mitochondrial function returns during hepatic recovery. Because the products of mitochondrial protein ...
Combined autoradiographic-immunocytochemical analysis of opioid receptors and opioid peptide neuronal systems in brain
(Elsevier, 1985)
Using adjacent section autoradiography-immunocytochemistry, the distribution of [3H]naloxone binding sites was studied in relation to neuronal systems containing [Leu]enkephalin, dynorphin A, or [beta]-endorphin immunoreactivity ...