Now showing items 21-30 of 45
A new negative control gene for amino acid biosynthesis in Saccharomyces cerevisiae
(Springer-Verlag, 1986-03)
Enzyme levels in multiple amino acid biosynthetic pathways in yeast are coregulated. This control is effected largely at the transcriptional level by a number. of regulatory genes. We report the isolation and characterization ...
Reinvestigation of the roles of the carboxyl groups of glutathione with yeast glyoxalase I : Implications as to the mechanism and coenzymic role of glutathione
(Elsevier, 1986-07-07)
A number of carboxyl-substituted s-blocked glutathiones have been shown to be competitive inhibitors of yeast glyoxalase I at 25[deg]C, pH 6.6. Amidation of the glycyl carboxyl group of S-(p-bromobenzyl)glutathione has no ...
Madin-Darby canine kidney cells display type I and type II insulin-like growth factor (IGF) receptors
(Elsevier, 1986-01-14)
Using affinity cross-linking techniques, we report the presence of type I IGF and type II IGF receptors in Madin-Darby canine kidney cells, a line of cells lacking insulin receptors. The IGF receptors were further characterized ...
Lack of first-pass metabolism of ethanol at blood concentrations in the social drinking range
(Elsevier, 1986-08-04)
Previously published data are displayed in a new manner and show that there is complete systemic availability of oral doses of 23-47 g of ethanol (0.35-0.75 g/kg or 30-60 ml of 95% ethanol) in man when administered in the ...
Evidence that the subunit structure of gonadotropin receptor is preserved during regression of rat corpus luteum
(Elsevier, 1986-05-29)
The level of hCG/LH receptor has been shown to undergo marked changes during the life span of rat corpus luteum. To evaluate whether these fluctnations are due to changes in the receptor subunit structure or receptor protein ...
GTP and cytosol stimulate phosphoinositide hydrolysis in isolated platelet membranes
(Elsevier, 1986-06-13)
Hydrolysis of polyphosphoinositides by phospholipase C was examined in isolated membranes prepared from [32P]labelled platelets. In the presence of GTP[gamma]S, thrombin increased the release of inositol triphosphate and ...
Discriminative and aversive properties of [beta]-carboline-3-carboxylic acid ethyl ester, a benzodiazepine receptor inverse agonist, in rhesus monkey
(Elsevier, 1986-03-17)
Rhesus monkeys were trained to discriminate injections of saline from those of [beta]-carboline-3-carboxylic acid ethyl ester ([beta]-CCE), a compound that binds to the benzodiazepine receptor, but often has actions opposite ...
Ontogenesis of corticotropes and lactotropes in situ in the pituitary gland of the hamster
(Springer-Verlag, 1986-09)
The development of corticotropes and lactotropes was investigated in the golden Syrian hamster using an anti-porcine ACTH antiserum and a homologous antihamster PRL antiserum. Oval corticotropes were first visible in the ...
Amino acid sequence of the active site of human serum cholinesterase from usual, atypical, and atypical-silent genotypes
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1986-06)
Active-site tryptic peptides were isolated from three genetic types of human serum cholinesterase. The active-site peptide was identified by labeling the active-site serine with [ 3 H] diisopropylfluorophosphate. Peptides ...
Tumor necrosis factor-induced alterations in circulating leukocyte populations
(Elsevier, 1986-12-15)
Tumor necrosis factor is a potent agent possessing diverse biological functions. We investigated the effects of intravenous administration of human recombinant tumor necrosis factor (TNF) on immune cell populations in CBA/J ...