Now showing items 41-50 of 61
Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli
(Elsevier, 1990-12)
DNA polymerase from Thermus aquaticus has become a common reagent in molecular biology because of its utility in DNA amplification and DNA sequencing protocols. A simplified method is described here for isolating the ...
Chemisorption and FTIR study of bimetallic Pt---Au/SiO₂catalysts
(Elsevier, 1990-02)
Pt/SiO₂, Au/SiO₂, and bimetallic Pt---Au/SiO₂ catalysts were prepared by incipient wetness impregnation of nonporous SiO₂. The catalysts were characterized after reduction in H₂ by static volumetric chemisorption and ...
The mechanism of the oxidation of propene to acrolein over antimony-tin mixed oxide catalysts
(Elsevier, 1990-05)
The oxidation of propenes such as 13CH2=CH---CH,3, CH2=CH-CD3, cis---CHD=CD---CH3, and CH2---CH---CH3 was studied over Sb6O13, SnO2, and Sb---Sn mixed oxide catalysts. The results with 13CH2=CH---CH3 and CH2=CH---CD3 were ...
Thermodynamics of the (lanthanum + strontium + copper + oxygen) high-Tc superconductors I. Heat capacities of SrCuO2, Sr2CuO3, and Sr14Cu24O41
(Elsevier, 1990-11)
The subambient heat capacities of three (SrO)x(CuO)y inter-oxide samples with x = Y = 1; x = 2, y = 1; and x = 1, y = 2, were measured as a part of an investigation into the chemical thermodynamics of the high-Tc superconducting ...
Glycosidases of ehrlich ascites tumor cells and ascitic fluid--Purification and substrate specificity of [alpha]-N-Acetylgalactosaminidase and [alpha]-galactosidase: Comparison with coffee bean [alpha]-galactosidase
(Elsevier, 1990-07)
Ehrlich ascites tumor cells and ascitic fluid were assayed for glycosidase activity. [alpha]-Galactosidase and [beta]-galactosidase, [alpha]- and [beta]-mannosidase, [alpha]-N-acetylgalactosaminidase, and [beta]-N-acetyl ...
(1E,3E)-4-acetoxy-1-phenyldimethylsilyl-1,3-butadiene as a surrogate for (1E,3E)-1,4- diacetoxy-1,3-butadiene: a highly efficient synthesis of (+/-)-shikimic acid
(Elsevier, 1990)
The 5-step synthesis of (+/-)-shikimic acid has been achieved in 55% overall yield from (1E,3E)-4-acetoxy-1-phenyldimethylsilyl-1,3-butadiene, starting with its Diels-Alder reaction with 2-(trimethylsilyl)ethyl acrylate ...
Kinetic studies of the deactivation of O 2 ( 1 Σ g + ) and O( 1 D)
(John Wiley & Sons, Inc., 1990-12)
The kinetics of the deactivation of O 2 ( 1 Σ g + ) is studied in real time. O 2 ( 1 Σ g + ) is generated in this system by the O( 1 D) + O 2 reaction following O 3 laser flash photolysis in the presence of excess ...
Inhibition of cytotoxicity by intracellular superoxide dismutase supplementation
(Elsevier, 1990)
The role of intracecullar oxyradicals in H2O2 and neutrophil-induced cytotoxicity is suggested by previous studies showing protection by inhibitors such as deferroxamine, dimethylthiourea, and dimethyl sulfoxide. In the ...
Simplified method of making alginate-polylysine microcapsules for hybridoma cell culture using RPMI 1640 medium
(Kluwer Academic Publishers; Science & Technology Letters ; Springer Science+Business Media, 1990-09)
The method of making alginate-poly-L-lysine microcapsules for hybridoma cell culture can be simplified by cultivating the cells in RPMI 1640 medium. Phosphate concentration in RPMI 1640 medium is sufficiently high to ...