Now showing items 11-20 of 44
An improved method for immobilizing IgG antibodies on protein A-agarose
(Elsevier, 1990-03-09)
This report describes a modification of a procedure developed by others for crosslinking IgG to protein A which itself is covalently linked to a gel support. Earlier immunoaffinity columns were described as having large ...
Immobilization can improve the stability of hybridoma antibody productivity in serum-free media
(Wiley Subscription Services, Inc., A Wiley Company, 1990-12-05)
Hybridoma cells (S3H5/Γ2bA2) were cultivated in spinner flasks with 1% serum media and serum-free media. Monoclonal antibody productivity was maintained in 1% serum media. However, cells in serum-free media showed a decrease ...
Feedback regulation and the intracellular protein profile of Streptomyces griseus in a cycloheximide fermentation
(Springer-Verlag, 1990-11)
Two-dimensional gel electrophoresis (2-D PAGE) was used to study the intracellular protein profile of Streptomyces griseus in relation to cycloheximide (CH) biosynthesis. Four proteins (CR1-CR4) were found to be significantly ...
Adduct formation identification between phenyl glycidyl ether and 2'-deoxyadenosine and thymidine by chromatography, mass spectrometry and nuclear magnetic resonance spectroscopy
(Elsevier, 1990)
Thymidine 2'-deoxyadenosine were reacted with phenyl glycidyl ether in order to study the formation of the corresponding 2'-deoxynucleoside adducts. Separation methods were elaborated using either reversed-phase high-performance ...
Effector phenotype and immunologic specificity of T cell-mediated adoptive therapy for a murine tumor that lacks intrinsic immunogenicity
(Elsevier, 1990-08)
The MCA 102 sarcoma has been defined by a variety of immunologic studies as a tumor lacking intrinsic immunogenicity. Nevertheless, we have recently demonstrated the feasibility of generating therapeutically effective ...
Role of O2- in neutrophil recruitment into sites of dermal and pulmonary vasculitis
(Elsevier, 1990)
Using analogous models of acute dermal vasculities and alveolitis in rats, we have examined the role of oxygen-derived metabolities in the tissue damage associated with neutrophil influx into sites of immune complex ...
Global systems approach to bacterial physiology: protein responders to stress and starvation
(Elsevier, 1990-12-01)