Now showing items 31-40 of 44
A three-dimensional model to analyze drug-drug interactions
(Elsevier, 1990)
Nearly four generations of investigators have studied combined drug effects. Their methods of generating and analyzing data have changed dramatically over the years but the basic problem has not. This review examines the ...
A microtiter virus yield reduction assay for the evaluation of antiviral compounds against human cytomegalovirus and herpes simplex virus
(Elsevier, 1990-04)
Although the virus yield reduction assay is a powerful technique for evaluating the efficacy of antiviral compounds, it is not routinely utilized due to its labor-intensive nature. This procedure was modified, developed, ...
Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli
(Elsevier, 1990-12)
DNA polymerase from Thermus aquaticus has become a common reagent in molecular biology because of its utility in DNA amplification and DNA sequencing protocols. A simplified method is described here for isolating the ...
Glycosidases of ehrlich ascites tumor cells and ascitic fluid--Purification and substrate specificity of [alpha]-N-Acetylgalactosaminidase and [alpha]-galactosidase: Comparison with coffee bean [alpha]-galactosidase
(Elsevier, 1990-07)
Ehrlich ascites tumor cells and ascitic fluid were assayed for glycosidase activity. [alpha]-Galactosidase and [beta]-galactosidase, [alpha]- and [beta]-mannosidase, [alpha]-N-acetylgalactosaminidase, and [beta]-N-acetyl ...
Inhibition of cytotoxicity by intracellular superoxide dismutase supplementation
(Elsevier, 1990)
The role of intracecullar oxyradicals in H2O2 and neutrophil-induced cytotoxicity is suggested by previous studies showing protection by inhibitors such as deferroxamine, dimethylthiourea, and dimethyl sulfoxide. In the ...
Antigenic and functional analysis of a neutralization site of HSV-1 glycoprotein D
(Elsevier, 1990-02)
Herpes simplex virus glycoprotein D is a component of the virion envelope and appears to be involved in attachment, penetration, and cell fusion. Monoclonal antibodies (MAbs) against this protein can be arranged in groups, ...
In vivo evidence that the rise in plasma IL 6 following injection of a fever-inducing dose of LPS is mediated by IL 1[beta]
(Elsevier, 1990-05)
Although it has often been speculated that Interleukin (IL) 1[alpha] and IL 1[beta] are circulating endogenous pyrogens (EP), there are few data demonstrating an elevation of these cytokines in the plasma of febrile animals. ...
The participation of coenzyme Q in free radical production and antioxidation
(Elsevier, 1990)
Published experimental data pertaining to the participation of coenzyme Q as a site of free radical formation in the mitochondrial electron transfer chain and the conditions required for free radical production have been ...