Now showing items 21-30 of 78
Glycine-extended post-translational processing intermediates of gastrin and cholecystokinin in the gut
(Elsevier, 1994-02-03)
Cholecystokinin (CCK) and gastrin are two polypeptide hormones of the gut that share complete structural homology in their carboxyl-terminal pentapeptide. Both peptides are biologically activated from their glycine-extended ...
Frequent harvesting from perfused bone marrow cultures results in increased overall cell and progenitor expansion
(Wiley Subscription Services, Inc., A Wiley Company, 1994-08-20)
The establishment of prolific long-term human bone marrow cultures has led to the development of hematopoietic bioreactor systems. A single batch expansion of bone marrow mononuclear cell populations leads to a 10- to ...
Microencapsulated human bone marrow cultures: A potential culture system for the clonal outgrowth of hematopoietic progenitor cells
(Wiley Subscription Services, Inc., A Wiley Company, 1994-04-05)
Currently the most successful methods for culturing human hematopoietic cells employ some form of perfused bioreactor system. However, these systems do not permit the clonal outgrowth of single progenitor cells. Therefore, ...
Differential expression of metabotropic glutamate receptors in the hippocampus and entorhinal cortex of the rat
(Elsevier, 1994-02)
Metabotropic glutamate receptors (mGluRs) have been implicated in a number of hippocampal functions including learning and memory. Five subtypes have been molecularly and pharmacologically characterized. Using in situ ...
tRNA-guanine transglycosylase from Escherichia coli: Recognition of dimeric, unmodified tRNATyr
(Elsevier, 1994)
In order to probe the interaction between tRNA and the tRNA hypermodifying enzyme, tRNA-guanine transglycosylase (TGT) from Escherichia coli, we have undertaken the generation of E coli tRNATyr and analogues. During efforts ...
Corticotropin releasing hormone is involved in exercise-induced elevation in core temperature
(Elsevier, 1994)
To determine the involvement of corticotropin releasing factor (CRF) in exercise-induced elevation in core temperature of female rats, CRF antibody or vehicle was injected intracerebroventricularly (ICV) into rats that had ...
Whole skeletal muscle transplantation: Mechanisms responsible for functional deficits
(Wiley Subscription Services, Inc., A Wiley Company, 1994-04-05)
One aspect of tissue engineering of skeletal muscle involves the transposition and transplantation of whole muscles to treat muscles damaged by injury or disease. The transposition of whole muscles has been used for many ...
Expression of Corticotropin-Releasing Hormone Transgenes in Neurons of Adult and Developing Mice
(Elsevier, 1994-12)
The DNA sequences important for cell-specific expression and developmental regulation of corticotropin-releasing hormone (CRH) were analyzed in transgenic mice. A construct containing 0.5 kb of CRH 5' flanking DNA linked ...
Application of the gel shift assay to study the affinity and specificity of anti-DNA autoantibodies
(Elsevier, 1994-12-28)
We have demonstrated that the gel shift assay, a powerful method to study protein [middle dot] DNA interactions under equilibrium conditions, is both an accurate and precise method to measure the affinity of anti-DNA [middle ...