Now showing items 21-30 of 36
Probing RNA Structure and Metal‐Binding Sites Using Terbium(III) Footprinting
(John Wiley & Sons, 2003-06)
The function of an RNA molecule is determined by its overall secondary and tertiary structure. The tertiary structure is facilitated and stabilized by the interaction with metal ions. The current chapter offers a detailed ...
Evaluating hydrogen bond interactions in enzymes containing Mn(III)-histidine complexation using manganese-imidazole complexes
(Springer-Verlag; SBIC, 2003-02)
It is often difficult to control hydrogen bond interactions in small molecule compounds that model metalloenzyme active sites. The imidazole-containing ligands 4,5-dicarboxyimidazole (H 3 DCBI) and 4,5-dicarboxy- N ...
Capillary Electrophoresis of DNA
(John Wiley & Sons, 2003-06)
Capillary electrophoresis (CE) is an alternative to conventional slab gel electrophoresis for the separation of DNA fragments. CE offers a number of advantages over slab gel separations in terms of speed, resolution, ...
Synthesis of Oligonucleotide Conjugates via Aqueous Diels‐Alder Cycloaddition
(Wiley Periodicals, Inc.Cold Spring Harbor Laboratory Press, 2003-09)
The conjugation of maleimide reporter groups to 5′‐diene‐modified oligonucleotides via aqueous Diels‐Alder cycloaddition is described. Detailed basic protocols are provided for the preparation of a diene‐amidite (5′‐diene ...
Potential energy functions: From consistent force fields to spectroscopically determined polarizable force fields
(Wiley Subscription Services, Inc., A Wiley Company, 2003-03)
We review our methodology for producing physically accurate potential energy functions, particularly relevant in the context of Lifson's goal of including frequency agreement as one of the criteria of a self-consistent ...
Pharmacophore and receptor models for neurokinin receptors
(Kluwer Academic Publishers; Springer Science+Business Media, 2003-11)
Three neurokinin (NK) antagonist pharmacophore models (Models 1–3) accounting for hydrogen bonding groups in the `head' and `tail' of NK receptor ligands have been developed by use of a new procedure for treatment of ...
Synthesis of 1,5‐Anhydrohexitol Building Blocks for Oligonucleotide Synthesis
(Wiley Periodicals, Inc., 2003-09)
This unit describes in detail, the optimized preparations of 1,5‐anhydrohexitol and the 1,5‐anhydrohexitol building blocks for oligonucleotide synthesis (hG, hA, hC, hT).
Uridine 2′‐Carbamates: Facile Tools for Oligonucleotide 2′‐Functionalization
(Wiley Periodicals, Inc., 2003-12)
A facile method for preparation of uridine 2′‐carbamate derivatives based on reaction of 3′,5′‐disilyl‐protected uridine with 1,1′‐carbonyldiimidazole followed by treatment with an aliphatic amine is presented. A phosphoramidite ...
A novel discrete dinuclear copper(II)–gadolinium(III) complex derived from a Schiff base ligand [Cu(salbn)Gd(NO 3 ) 3 ·H 2 O] (salbn): N , N ′-butylenebis(salicylideaminato)
(Kluwer Academic Publishers; Springer Science+Business Media, 2003-09)
The synthesis, X-ray and e.p.r. spectral studies of a 3d–4f couple are described here. The crystal structure of [Cu(salbn)Gd(NO 3 ) 3 ·H 2 O], (2) , salbn = N , N ′-butylenebis(salicylideaminato), has been determined by ...
The effect of fiber orientation on the toughening of short fiber-reinforced polymers
(Wiley Subscription Services, Inc., A Wiley Company, 2003-12-05)
The effect of fiber orientation on the toughening of polymers by short glass fibers generally below their critical length was investigated using specimens with either well-aligned or randomly oriented fibers. The fibers ...