Now showing items 1-10 of 46
Rapid protein release from Escherichia coli by chemical permeabilization under fermentation conditions
(Wiley Subscription Services, Inc., A Wiley Company, 1992-03-25)
Overall protein release greater than 75% in less than 1 h can be attained by exposing exponentially growing Escherichia coli cells to 0.4 M guanidine plus 0.5% Triton X-100 at 37°C in medium. Cell growth stops immediately ...
On performing experimental studies on transient states of continuous-flow methanogenic reactors
(Wiley Subscription Services, Inc., A Wiley Company, 1990-03-25)
One approach to exploring the behavior of microbial cultures during transient conditions of unbalanced growth is to experimentally observe continuous-flow biological reactors which have been subjected to perturbations in ...
Quantitative evaluation of the pH profile in organic acid fermentations
(Wiley Subscription Services, Inc., A Wiley Company, 1991-01-20)
No Abstrct.
Selective extraction using preferential transport through adsorptive membranes
(Wiley Subscription Services, Inc., A Wiley Company, 1996-12-05)
Selective extraction of a protein from a mixture can be accomplished using an adsorptive membrane and low displacement recuperative parametric pumping. Low displacement recuperative parametric pumping can lead to the ...
Reproduction numbers and thresholds in stochastic epidemic models I. Homogeneous populations
(Elsevier, 1991-12)
We compare threshold results for the deterministic and stochastic versions of the homogeneous SI model with recruitment, death due to the disease, a background death rate, and transmission rate [beta]cXY / N. If an infective ...
Physiological changes during the adaptation of hybridoma cells to low serum and serum-free media
(Wiley Subscription Services, Inc., A Wiley Company, 1991-01-05)
Two murine hybridoma cell lines (167.4G5.3 and S3H5/Γ2bA2) were adapted to grow in low-serum and serum-free media by a weaning procedure. The changes in cell growth, metabolic, and antibody production rates with adaptation ...
Population balance between producing and nonproducing hybridoma clones is very sensitive to serum level, state of inoculum, and medium composition
(Wiley Subscription Services, Inc., A Wiley Company, 1992-02-05)
Secreting and nonsecreting hybridoma populations derived from the murine hybridoma cell line 167.4G5.3 were each grown in batch culture in low serum and serum-free media. Under serum-free conditions, a secreting population ...
Development of pure culture biofilms of P. putida on solid supports
(Wiley Subscription Services, Inc., A Wiley Company, 1991-03-15)
Pseudomonas putida biofilms were developed on and biofilm accumulation rate data were obtained for the following two classes of support materials: charged surfaces and noncharged hydrophobic and hydrophilic surfaces. The ...
Elemental balancing of biomass and medium composition enhances growth capacity in high-density Chlorella vulgaris cultures
(Wiley Subscription Services, Inc., A Wiley Company, 1998-09-05)
The basic requirements for high-density photoautotrophic microalgal cultures in enclosed photobioreactors are a powerful light source and proper distribution of light, efficient gas exchange, and suitable medium composition. ...
Protein extraction using the sodium bis(2-ethylhexyl) phosphate (NaDEHP) reverse micellar system
(Wiley Subscription Services, Inc., A Wiley Company, 1996-04-20)
The reverse micellar system of sodium bis(2-ethylhexyl) phosphate (NaDEHP)/isooctane/brine was used for liquid-liquid extraction of proteins. We investigated the solubilization of cytochrome-c and Α-chymotrypsin into the ...