Now showing items 91-100 of 235
Derivatization of Glass and Polypropylene Surfaces
(Wiley Periodicals, Inc., 2004-09)
This unit describes the derivatization of solid support media, particularly glass and polypropylene, with a linker system by an iterative process that forms a dendrimeric structure, thereby increasing stepwise the loading ...
Synthesis and Characterization of Chimeric 2‐5A‐DNA Oligonucleotides
(Wiley Periodicals, Inc.Elsevier/ North‐Holland, 2000-07)
This unit provides protocols for the synthesis and characterization of 2‐5A‐antisense nucleic acids. These chimeric oligonucleotides consist of 2′,5′‐phosphodiester‐linked oligoadenylates ligated to 3′,5′‐deoxyribonucleotides ...
Proteomic Analysis Using 2‐D Liquid Separations of Intact Proteins From Whole‐Cell Lysates
(Marcel DekkerWiley Periodicals, Inc., 2003-11)
This unit describes procedures for 2‐D liquid separations of proteins from whole‐cell lysates. Protocols for protein isoelectric point (pI) fractionation in the first dimension include the use of liquid isoelectric focusing ...
Aluminum(III) Porphyrins as Ionophores for Fluoride Selective Polymeric Membrane Electrodes
(WILEY-VCH Verlag, 2006-03)
Aluminum(III) porphyrins are examined as potential fluoride selective ionophores in polymeric membrane type ion-selective electrodes. Membranes formulated with Al(III) tetraphenyl (TPP) or octaethyl (OEP) porphyrins are ...
Synthesis of Endcap Dimethoxytrityl Phosphoramidites for Endcapped Oligonucleotides
(Wiley Periodicals, Inc., 2003-03)
Endcaps may be either aromatic or aliphatic molecules that specifically crossâ link the 5â ² end of one strand with the 3â ² end of the complementary strand in a DNA duplex. Endcaps may be viewed as a replacement of the ...
Glutathione Peroxidase-Based Amperometric Biosensor for the Detection of S -Nitrosothiols
(WILEY-VCH Verlag, 2006-11)
A new biosensor is described for the detection of S -nitrosothiols (RSNOs) based on their decomposition by immobilized glutathione peroxidase (GPx), an enzyme containing selenocysteine residue that catalytically produces ...
Selected Abbreviations Used in This Manual
(Wiley Periodicals, Inc., 2004-09)
This APPENDIX provides commonly used abbreviations for many terms used in in this manual.