Now showing items 101-110 of 235
Overview of the Synthesis of Nucleoside Phosphates and Polyphosphates
(Wiley‐Interscience, 2003-12)
This overview summarizes methodology used for the synthesis of nucleoside mono‐, di‐, and triphosphates. Selected techniques such as the Mitsunobu reaction, displacement reactions involving nucleoside 5′‐tosylates, ...
Reversible Biotinylation of the 5′‐Terminus of Oligodeoxyribonucleotides and its Application in Affinity Purification
(John Wiley & Sons, 2003-09)
The preparation of two reversible biotinylation phosphoramidites and their application in labeling and affinity purification of synthetic oligodeoxyribonucleotides will be described. In both cases, the biotin is linked to ...
Polyacrylamide Gel Electrophoresis (PAGE) of Synthetic Nucleic Acids
(Foster CityWiley Periodicals, Inc., 2000-07)
Protocols are given for analysis of oligonucleotides by PAGE, using either methylene blue staining or radiolabeling to mark the oligonucleotide. In addition, a separate protocol is provided for purification by PAGE.
Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20
(WILEY-VCH Verlag, 2006-05)
Chronic exercise training elicits adaptations in the heart that improve pump function and confer cardioprotection. To identify molecular mechanisms by which exercise training stimulates this favorable phenotype, a proteomic ...
2′‐Hydroxyl‐Protecting Groups that are Either Photochemically Labile or Sensitive to Fluoride Ions
(Wiley Periodicals, Inc., 2000-12)
Protected ribonucleotide monomers are more difficult to obtain than their 2′‐deoxy counterparts because of the need to protect the 2′‐hydroxy function. This unit describes the stepwise preparation of suitably 2′‐protected ...
Proteome-wide changes induced by the Hsp90 inhibitor, geldanamycin in anaplastic large cell lymphoma cells
(WILEY-VCH Verlag, 2007-08)
The molecular chaperone heat shock protein 90 (Hsp90) affects the function of many oncogenic signaling proteins including nucleophosmin-anaplastic lymphoma kinase (NPM-ALK) expressed in anaplastic large cell lymphoma (ALCL). ...
Interaction between gemcitabine and mitomycin-C in vitro
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 2000-01)
Both gemcitabine (2′,2′-difluorodeoxycytidine; dFdCyd) and mitomycin-C (MMC) are active against several solid malignancies. dFdCyd is an attractive agent for use in combination with drugs which damage DNA and with radiation ...
Analysis of Oligonucleotides by Matrixâ Assisted Laser Desorption/Ionization Timeâ ofâ Flight Mass Spectrometry
(Wiley Periodicals, Inc., 2000-02)
MALDIâ MS is one of the most useful techniques available for determining biomolecule mass. It offers high mass accuracy, good sensitivity, simplicity, and speed. Because singly charged ions of oligonucleotides are typically ...
5′‐Iodination of Solid‐Phase‐Linked Oligodeoxyribonucleotides
(Wiley Periodicals, Inc., 2003-09)
5′‐Iodinated oligodeoxyribonucleotides readily react with 3′‐phosphorothioated DNA in the presence of a complementary template to yield a conjugate that is identical to natural DNA in every respect except that one oxygen ...
Probing RNA Structure and Metal‐Binding Sites Using Terbium(III) Footprinting
(John Wiley & Sons, 2003-06)
The function of an RNA molecule is determined by its overall secondary and tertiary structure. The tertiary structure is facilitated and stabilized by the interaction with metal ions. The current chapter offers a detailed ...