Now showing items 811-820 of 897
Inhibiting the Actions of Essential Biomolecule Phosphopantetheine.
(2015)
Phosphopantetheine is an essential biomolecule required for life. The enzyme phosphopantothenoylcysteine synthetase (PPCS) incorporates the reactive thiol moiety in the Coenzyme A (CoA) biosynthetic pathway. Two types of ...
Novel Modulators of Rho Transcriptional Signaling in Cancer.
(2012)
Rho A and Rho C, members of the Rho GTPase family, have been found to have important roles in the metastases of multiple cancers, including melanoma, prostate, and breast cancer. While Rho GTPases are known to have effects ...
Dissecting the Roles of the Transcriptional Coactivator p300 Regulating the Oncogenic Self-Renewal Gene NANOG.
(2014)
Eukaryotic gene expression is controlled by the concerted actions and interactions of DNA-regulatory elements, transcriptional activators and associated coactivators. Successful assembly of the proper activator and ...
Structural and Biochemical Analysis of G Protein-Coupled Receptor Kinase Activation and Small Molecule Inhibitor Selectivity
(2019)
Cellular signaling through G protein-coupled receptors (GPCRs) is essential for most physiological processes, ranging from vision, taste, and smell to immune, neurological, and cardiovascular functions. In order to remain ...
Investigating Endothelial Dysfunction in a CRISPR/Cas9 Model of Fabry Disease
(2017)
Fabry disease is a rare, X-linked lysosomal storage disease arising from deficiency of the lysosomal hydrolase, α-galactosidase A (GLA). Reduced GLA activity disrupts glycosphingolipid (GSL) catabolism, leading to widespread ...
Discovery and Development of Small Molecule Probes for the SUMO protease SENP1, a Novel Target for Advanced Prostate Cancer Therapy
(2017)
Androgen signaling through the androgen receptor (AR) is essential for normal growth and function of the prostate gland. In prostate cancer (PC), androgens provide the main proliferative drive for the disease, making ...
(1) The Relationship of Protein Expression and Cell Division, (2) 3D Imaging of Cells Using Digital Holography, and (3) General Chemistry Enrollment at University of Michigan.
(2012)
Chapter 1: We evaluated the relationship between cell division and protein expression when using commercial poly(ethylenimine) (PEI)-based polyplexes. The membrane dye PKH26 was used to assess cell division, and cyan ...
Diversity in Intracellular MicroRNA Regulatory Networks: MicroRNA-21 and Beyond.
(2012)
microRNAs (miRNAs) comprise a broad class of short non-coding RNA that regulate gene expression by guiding the RNA induced silencing complex (RISC) to mRNAs containing complementary ‘seed sites’. General principles of miRNA ...
Elucidating the Potential Role of Protein Prenylation in Bacterial Infection.
(2016)
Many G-proteins, like Ras, require prenylation for membrane localization and function. Protein farnesyltransferase (FTase) and geranylgeranyltransferase type I (GGTase-I) add 15-carbon farnesyl groups and 20-carbon ...
Characterization of the Protein-Protein Interactions between Histone Methyltransferase DOT1L and MLL Fusion Proteins towards Developing Small Molecule Inhibitors.
(2013)
The MLL fusion proteins activate target genes in part via recruitment of DOT1L (disruptor of telomeric silencing 1-like), a histone H3 lysine 79 (H3K79) methyltransferase. The resulting hypermethylation of H3K79 at the Hox ...