Now showing items 851-860 of 897
Directed Evolution of Stabilized Peptides with Bacterial Display
(2020)
Interactions between proteins govern cellular and the body’s states, including aberrant interactions found in diseases such as in cancers and infections. Small molecule drugs are not ideal in targeting these interactions ...
Investigating Protein-Membrane Interactions Mediated by Factor X That Regulate the Blood Clotting Cascade
(2022)
Vascular trauma triggers the blood clotting cascade to maintain hemostasis by sequentially converting several zymogens to active enzymes via limited proteolysis. Coagulation factor X (FX) is activated by the extrinsic and ...
Exploring the Function of Polyphosphate in the Contact Pathway of Blood Clotting and Developing Polyphosphate Probes for Enhanced Specificity
(2021)
The classic coagulation cascade has been well-studied for many years. In the traditional “waterfall” model, both the contact pathway and the tissue factor pathway can trigger blood clotting. The contact pathway is composed ...
Characterization of Methyltransferase Carbon-Oxygen Hydrogen Bonding and Sulfur-Oxygen Chalcogen Bonding with the Sulfonium of S-adenosyl-L-methionine
(2018)
Methyltransferases use S-adenosyl-L-methionine (AdoMet) to transfer the one-carbon group to nitrogen, carbon, oxygen, and sulfur nucleophiles, among other atoms. AdoMet is the most common biological methyl donor, and the ...
Functional Characterization of Fibrinolysis-Modulating Proteins Using High Throughput Phage Display
(2020)
The advent of modern high throughput sequencing (HTS) technology has resulted in the resurgence of phage display as a powerful technique in screening peptide and protein libraries for mutations and/or protein fragments ...
UDP-Glucose: Glycoprotein Glucosyltransferase (UGGT1) Promotes Substrate Solubility in the Endoplasmic Reticulum.
(2015)
Protein folding in the endoplasmic reticulum (ER) is error-prone, and ER quality control processes exist (ERQC) to ensure only correctly folded proteins are exported from the ER en route to the Golgi compartment. Glycoproteins ...
The Role of Collagen Cross-linking in Craniofacial and Long Bone Mineralization and Healing
(2021)
Compromised collagen and mineral can lead to decreases in bone quantity and quality in a variety of diseases that differentially affect craniofacial and long bones. Since bone is a composite - with mineral lending stiffness ...
Development, Synthesis, and Characterization of G Protein-Coupled Receptor Kinase Inhibitors Using Structure Based Drug Design for the Advancement of Heart Failure Therapeutics
(2017)
In heart failure, the β-adrenergic receptors (βARs) become desensitized and uncoupled from heterotrimeric G proteins. This process is initiated by G protein-coupled receptor kinases (GRKs), some of which are upregulated ...
An Investigation of the RNA Induced Silencing Complex and its Therapeutic Implications.
(2013)
Small interfering RNAs (siRNAs) are regulatory non-coding RNA, which regulate gene expression at the post-transcriptional level through the highly conserved RNA interference (RNAi) pathway. RNAi is employed by most eukaryotes ...
The Function of the ASH1L Histone Methyltransferase in Cancer: A Chemical Biology Approach
(2018)
ASH1L (absent, small, or homeotic-like 1) is a histone lysine methyltransferase (KMTase) that is overexpressed in cancer and activates oncogenic HOX genes. Small molecule inhibitors of ASH1L would be invaluable tools to ...