Now showing items 51-60 of 172
A general relation between membrane potential, ion activities, and pump fluxes for nonsymmetric cells in a steady state
(Elsevier, 1981-02)
In a previous paper Jacquez and Schultz [Math. Biosci. 20:19 (1974)] derived a set of relations between the membrane potential, ion activities, ion permeabilities, and mediated ion fluxes which held for steady states of ...
1-Monoglyceride production from lipase-catalyzed esterification of glycerol and fatty acid in reverse micelles
(Wiley Subscription Services, Inc., A Wiley Company, 1991-08-20)
Glycerol-fatty acid esterification has been conducted with lipase from R. delemar in water/AOT/isooctane reverse micellar media, with the major product being 1-monoglyceride, a useful food-emulsifier. 1,3-diglyceride was ...
Validation of a two-parameter quantitative structure–activity relationship as a legitimate tool for rational re-design of horseradish peroxidase
(Wiley Subscription Services, Inc., A Wiley Company, 2007-09-01)
Previously reported rates of reaction between six mutant strains of the enzyme horseradish peroxidase (HRP) and a test substrate, 2-methoyxpyhenol, were found to correlate with characteristic binding distances computed ...
Frequent harvesting from perfused bone marrow cultures results in increased overall cell and progenitor expansion
(Wiley Subscription Services, Inc., A Wiley Company, 1994-08-20)
The establishment of prolific long-term human bone marrow cultures has led to the development of hematopoietic bioreactor systems. A single batch expansion of bone marrow mononuclear cell populations leads to a 10- to ...
Mold‐casted non‐degradable, islet macro‐encapsulating hydrogel devices for restoration of normoglycemia in diabetic mice
(Wiley Periodicals, Inc., 2016-11)
Islet transplantation is a potential cure for diabetic patients, however this procedure is not widely adopted due to the high rate of graft failure. Islet encapsulation within hydrogels is employed to provide a three‐dimensional ...
Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production
(Wiley Subscription Services, Inc., A Wiley Company, 1992-02-20)
The influence of ammonia and lactate on cell growth, metabolic, and antibody production rates was investigated for murine hybridoma cell line 163.4G5.3 during batch culture. The specific growth rate was reduced by one-half ...
Microencapsulated human bone marrow cultures: A potential culture system for the clonal outgrowth of hematopoietic progenitor cells
(Wiley Subscription Services, Inc., A Wiley Company, 1994-04-05)
Currently the most successful methods for culturing human hematopoietic cells employ some form of perfused bioreactor system. However, these systems do not permit the clonal outgrowth of single progenitor cells. Therefore, ...
Dynamic microRNA activity identifies therapeutic targets in trastuzumab‐resistant HER2+ breast cancer
(Wiley Periodicals, Inc.Springer, 2018-10)
MicroRNAs (miRNAs) are implicated in numerous physiologic and pathologic processes, such as the development of resistance to chemotherapy. Determining the role of miRNAs in these processes is often accomplished through ...
Analysis of double-substrate limited growth
(Wiley Subscription Services, Inc., A Wiley Company, 1978-02)
Mathematical models which relate the growth rate of a microorganism to a single limiting substrate concentration have long been established. In recent years, it has become apparent that, under certain conditions, the growth ...
Optimizing seeding and culture methods to engineer smooth muscle tissue on biodegradable polymer matrices
(Wiley Subscription Services, Inc., A Wiley Company, 1998-01-05)
The engineering of functional smooth muscle (SM) tissue is critical if one hopes to successfully replace the large number of tissues containing an SM component with engineered equivalents. This study reports on the effects ...