Now showing items 61-70 of 169
Loss of antibody productivity is highly reproducible in multiple hybridoma subclones
(Wiley Subscription Services, Inc., A Wiley Company, 1993-06-20)
An immunoglobulin G (IgG 2b ) producing hybridoma cell line (S3H5/Γ2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum-free medium. Following adaptation ...
Fermentation monitoring by polymeric-resin-based microbiol assay
(Wiley Subscription Services, Inc., A Wiley Company, 1983-03)
A polymeric-resin-based microbiological assay is shown to yield results consistent with classical inhibition zone theory. This novel assay is applicable to cycloheximide, an antifungal antibiotic, at concentrations below ...
Recreating the perivascular niche ex vivo using a microfluidic approach
(Wiley Subscription Services, Inc., A Wiley Company, 2010-12-15)
Stem cell niches are composed of numerous microenvironmental features, including soluble and insoluble factors, cues from other cells, and the extracellular matrix (ECM), which collectively serve to maintain stem cell ...
Cell immobilization in k -carrageenan with tricalcium phosphate
(Wiley Subscription Services, Inc., A Wiley Company, 1982-08)
The objectives of this research were to investigate the growth of immobilized yeast cells in k -carrageenan gel and study the effect of trapping hydroxyapatite (tricalcium phosphate) crystals into the matrix. Using k ...
Combinatorial lentiviral gene delivery of pro‐oligodendrogenic factors for improving myelination of regenerating axons after spinal cord injury
(Wiley Periodicals, Inc., 2019-01)
Spinal cord injury (SCI) results in paralysis below the injury and strategies are being developed that support axonal regrowth, yet recovery lags, in part, because many axons are not remyelinated. Herein, we investigated ...
Coupling of concanavalin A to cellulose hollow fibers for use in glucose affinity sensor
(Wiley Subscription Services, Inc., A Wiley Company, 1986-02)
This article describes a method for the chemical immobilization of concanavalin A (Con A) on the inside wall of a single hollow cellulose fiber for use in glucose affinity sensor. Periodate oxidation of cellulose fiber ...
Countercurrent gradient chromatography: A continuous focusing technique
(Wiley Subscription Services, Inc., A Wiley Company, 1995-12-05)
The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin ...
Biochemical systems theory and metabolic control theory: 2. the role of summation and connectivity relationships
(Elsevier, 1987-10)
Perhaps the major obstacle to recognizing the relatedness of Biochemical Systems Theory (BST) and a subsequently developed approach some have called Metabolic Control Theory (MCT) is the summation and connectivity ...
Inhibitory effects of the macrolide antimicrobial tylosin on anaerobic treatment
(Wiley Subscription Services, Inc., A Wiley Company, 2008-09-01)
A laboratory-scale anaerobic sequencing batch reactor (ASBR) was operated using a glucose-based synthetic wastewater to study the effects of tylosin, a macrolide antimicrobial commonly used in swine production, on treatment ...