Now showing items 11-20 of 260
Retrievable hydrogels for ovarian follicle transplantation and oocyte collection
(American Cancer SocietyWiley Periodicals, Inc., 2018-08)
Cancer survivorship rates have drastically increased due to improved efficacy of oncologic treatments. Consequently, clinical concerns have shifted from solely focusing on survival to quality of life, with fertility ...
Cellular Delivery of Locked Nucleic Acids (LNAs)
(Wiley Periodicals, Inc., 2002-06)
Locked nucleic acids (LNAs) are RNA derivatives that have an O‐methylene linkage between the 2 and 4 positions of the ribose. This leads to exceptionally high‐affinity binding to complementary sequences. They are synthesized ...
Phospholipid compositional changes of five pseudomonad archetypes grown with and without toluene
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 2000-09)
Bacterial physiological responses to toluene exposure were investigated in five reference pseudomonad strains that express different toluene degradation pathways: Pseudomonas putida mt-2, Pseudomonas putida F1, Burkholderia ...
Base Composition Analysis of Nucleosides Using HPLC
(Applied Biosystems,Wiley Periodicals, Inc., 2000-07)
In this protocol, nuclease digestion of an oligonucleotide is followed by dephosphorylation and HPLC analysis of the monomers on a reversed‐phase C18 column. This method can be used to detect and quantitate a wide variety ...
Highly polarized T h17 cells induce EAE via a T ‐bet independent mechanism
(Wiley Periodicals, Inc., 2013-11)
Chemoenzymatic Preparation of Nucleoside Triphosphates
(Wiley Periodicals, Inc., 2004-03)
The design and synthesis of alternative nucleoside triphosphate substrates for DNA and RNA polymerases holds continued promise to create biochemical probes and precursors for synthesis of nucleic acid mimics. The azole ...
Modification of the 5′ Terminus of Oligonucleotides for Attachment of Reporter and Conjugate Groups
(John Wiley & Sons, 2000-02)
Reporter and conjugate groups can be added directly to the 5′ terminus of oligonucleotides by appropriate modification. Conjugate groups can be used to increase the affinity of complementary strands, induce irreversible ...
Mycobacterium avium Subspecies Paratuberculosis in the Inflamed Gut Tissues of Patients with Crohn’s Disease in China and its Potential Relationship to the Consumption of Cow’s Milk: A Preliminary Study
(Kluwer Academic Publishers; Springer, 2005-10)
Mycobacterium avium subspecies paratuberculosis infection in domestic livestock is widespread in many countries throughout the world. Studies in Europe and the USA show that M. avium subspecies paratuberculosis can be ...
Primary rat hepatocyte culture on 3D nanofibrous polymer scaffolds for toxicology and pharmaceutical research
(Wiley Subscription Services, Inc., A Wiley Company, 2011-01)
Primary rat hepatocytes are a widely used experimental model to estimate drug metabolism and toxicity. In currently used two-dimensional (2D) cell culture systems, typical problems like morphological changes and the loss ...
Protection of 2′‐Hydroxy Functions of Ribonucleosides
(Oxford University PressWiley Periodicals, Inc., 2000-02)
The main purpose of this article is to discuss 2′‐protection in the context of effective oligoribonucleotide synthesis. Emphasis is placed on the 2′‐protecting groups of choice in the synthesis of oligo‐and polyribonucleotides, ...