Now showing items 21-30 of 260
Microprinted feeder cells guide embryonic stem cell fate
(Wiley Subscription Services, Inc., A Wiley Company, 2011-10)
We introduce a non‐contact approach to microprint multiple types of feeder cells in a microarray format using immiscible aqueous solutions of two biopolymers. Droplets of cell suspension in the denser aqueous phase are ...
Synthesis and Purification of Oligonucleotide N3′→︀P5′ Phosphoramidates and their Phosphodiester and Phosphorothioate Chimeras
(Wiley Periodicals, Inc., 2000-12)
This unit describes the synthesis and purification of oligonucleotide N3′→︀P5′ phosphoramidates, wherein each 3′‐oxygen is replaced by a 3′‐amine in the 2′‐deoxyribose ring. The synthesis of required monomers and application ...
Site‐Specific Fluorescent Labeling of Large RNAs with Pyrene
(Oxford University PressWiley Periodicals, Inc., 2004-12)
Pyrene is a useful chromophore for monitoring the tertiary structure and folding of large RNAs. This unit describes the general preparation of a large RNA (>80 nucleotides in length) that has been site‐specifically modified ...
TLR3 modulates immunopathology during a Schistosoma mansoni egg-driven Th2 response in the lung
(WILEY-VCH Verlag, 2008-12)
We examined the role of TLR3 in Th2-driven pulmonary granulomatous disease, using wildtype (TLR3 +/+ ) and TLR3 gene-deficient (TLR3 −/− ) mice in a well-established model of Schistosoma mansoni egg-induced pulmonary ...
Preparation of 2′‐O‐[(Triisopropylsilyl)oxy]methyl‐protected Ribonucleosides
(Oxford University PressWiley Periodicals, Inc., 2001-12)
The [(triisopropylsilyl)oxy]methyl (TOM) group is a useful protecting group for the 2′‐OH of ribonucleosides to be used for oligoribonucleotide synthesis by the phosphoramidite method. It is completely stable to all reaction ...
Cell affinity separations using magnetically stabilized fluidized beds: Erythrocyte subpopulation fractionation utilizing a lectin-magnetite support
(Wiley Subscription Services, Inc., A Wiley Company, 2003-03-20)
A magnetically stabilized fluidized bed is used to separate erythrocyte subpopulations. Binding specificity was obtained by immobilizing the lectin Helix pomatia Agglutinin (HpA) or Griffonia simplicifolia I (GSI) onto ...
Synthesis of Phosphorothioate Oligonucleotides with Stereodefined Phosphorothioate Linkages
(Wiley‐Liss, 2003-09)
A method for solid‐phase synthesis of stereodefined PS‐oligos via an oxathiaphospholane approach using pure P‐diastereomers of nucleoside oxathiaphospholane monomers is described. The oxathiaphospholane monomers are ...
Evaluation of encapsulating and microporous nondegradable hydrogel scaffold designs on islet engraftment in rodent models of diabetes
(Wiley Periodicals, Inc., 2018-09)
Islet transplantation is a promising therapeutic option for type 1 diabetes mellitus, yet the current delivery into the hepatic portal vasculature is limited by poor engraftment. Biomaterials have been used as a means to ...