Now showing items 81-90 of 786
Synthesis and Purification of Oligonucleotide N3′→︀P5′ Phosphoramidates and their Phosphodiester and Phosphorothioate Chimeras
(Wiley Periodicals, Inc., 2000-12)
This unit describes the synthesis and purification of oligonucleotide N3′→︀P5′ phosphoramidates, wherein each 3′‐oxygen is replaced by a 3′‐amine in the 2′‐deoxyribose ring. The synthesis of required monomers and application ...
Development of adenovirus immobilization strategies for in situ gene therapy
(John Wiley & Sons, Ltd., 2008-10)
Background Regenerative gene therapy using viral vectors enables transduced cells to express bioactive factors in vivo . Viral delivery with spatial control can enhance transduction efficiency and may limit systemic ...
Quantitative Determination of High Charge Density Polyanion Contaminants in Biomedical Heparin Preparations Using Potentiometric Polyanion Sensors
(WILEY-VCH Verlag, 2010-01)
Quantification of oversulfated chondroitin sulfate (OSCS) in biomedical heparin preparations is achieved using a recently described potentiometric polyanion sensor-based approach operated in a kinetic mode of analysis. ...
Synthesis of the Tetraketide Lactones from the Pikromycin Biosynthetic Pathway
(WILEY-VCH Verlag, 2007-07)
Synthesis of tetraketide lactones 2 and 3 , which are likely to be produced by a model pikromycin polyketide synthase (PKS), has been investigated. The tetraketide lactones with six-membered rings, 2a and 2b , were ...
Site‐Specific Fluorescent Labeling of Large RNAs with Pyrene
(Oxford University PressWiley Periodicals, Inc., 2004-12)
Pyrene is a useful chromophore for monitoring the tertiary structure and folding of large RNAs. This unit describes the general preparation of a large RNA (>80 nucleotides in length) that has been site‐specifically modified ...
Adenosyl Radical: Reagent and Catalyst in Enzyme Reactions
(WILEY-VCH Verlag, 2010-03-22)
Adenosine is undoubtedly an ancient biological molecule that is a component of many enzyme cofactors: ATP, FADH, NAD(P)H, and coenzyme A, to name but a few, and, of course, of RNA. Here we present an overview of the role ...
TLR3 modulates immunopathology during a Schistosoma mansoni egg-driven Th2 response in the lung
(WILEY-VCH Verlag, 2008-12)
We examined the role of TLR3 in Th2-driven pulmonary granulomatous disease, using wildtype (TLR3 +/+ ) and TLR3 gene-deficient (TLR3 −/− ) mice in a well-established model of Schistosoma mansoni egg-induced pulmonary ...
Pleiotropy Analysis of Quantitative Traits at Gene Level by Multivariate Functional Linear Models
(John Wiley & Sons, 2015-05)
In genetics, pleiotropy describes the genetic effect of a single gene on multiple phenotypic traits. A common approach is to analyze the phenotypic traits separately using univariate analyses and combine the test results ...
Preparation of 2′‐O‐[(Triisopropylsilyl)oxy]methyl‐protected Ribonucleosides
(Oxford University PressWiley Periodicals, Inc., 2001-12)
The [(triisopropylsilyl)oxy]methyl (TOM) group is a useful protecting group for the 2′‐OH of ribonucleosides to be used for oligoribonucleotide synthesis by the phosphoramidite method. It is completely stable to all reaction ...