Now showing items 2031-2040 of 2090
Examination of Aggregation Prone Proteins and their Higher Order Interactions by Ion Mobility-Mass Spectrometry.
(2015)
Amyloid forming peptides and proteins present an extreme challenge for modern analytical measurement techniques. When natively-folded biomolecules partition into misfolded forms, they produce a myriad of inherently unstable ...
Evaluation and Development of Ligand-Dependent Fluorescent Reporters for Anaerobic Bacterial Imaging
(2021)
Fluorescence-based investigations of living cells and biological phenomena have been largely aided by the development of fluorescent proteins (FPs) to span a wide range of different colors and specialized photophysical ...
Combining Active Site and Allosteric Inhibitors to Study Protein Tyrosine Kinases
(2016)
Kinases play a crucial role in a variety of cell signaling pathways that control processes such as: growth, motility, and angiogenesis. Not surprisingly, dysregulation of proper kinase signaling often leads to cancer. Since ...
Development and Application of CDOCKER Docking Methodology
(2022)
The binding of small molecule ligands to receptor targets is important to numerous biological processes. When multiple ligands with different sizes are docked to a target receptor, it is reasonable to assume the residues ...
Ultraquantitative Raman Spectral Cytometry to Measure the Cargo Capacity of Individual Macrophages
(2019)
This dissertation demonstrates the usefulness of Raman microscopy as a multipurpose, 'high content' cytometric analysis tool for characterizing the phenotype of diverse macrophage populations in terms of their molecular ...
Developing Microfluidic Devices for the Optimization of Nanodiscs to Measure Membrane Protein Structure and Function
(2021)
Membrane proteins are essential to cellular functions, including cell-cell communication and signal transduction. However, the study of membrane proteins has lagged behind that of soluble proteins as once removed from the ...
Uncovering Hidden Dynamics in Living Systems Using Bayesian Statistics and Single-Molecule Microscopy
(2019)
Fluorescence microscopy is a powerful technique for understanding the organization, structure and dynamics of cells. Single-molecule imaging techniques extend our ability to probe cellular systems down to a range of only ...
Direct Identification and Counting of MicroRNAs in Single Cells by Transient Binding and Kinetic Fingerprinting
(2023)
Cancer is the second leading cause of mortality worldwide. Within the U.S. alone, it is estimated that 1.9 million people will be diagnosed this year and over 610,000 people are expected to perish. Despite advances in ...
Innovations in Droplet Microfluidics for Enzyme Evolution
(2020)
High throughput screening (HTS) is a critical technology in discovery and process chemistry. Typically, HTS has relied on robotic handling of well plates of samples to rapidly perform and assess reactions, but novel ...
Structure and Biochemistry of Cytochromes P450 Involved in the Biosynthesis of Macrolide Antibiotics
(2017)
Selective functionalization of chemically inert carbon-hydrogen (C–H) bonds embodies one of the grand challenges of organic chemistry and provides a key focus of research in the field. C–H functionalization can provide a ...