Now showing items 101-110 of 165
RNA Secondary Structure Prediction
(Kluwer Academic PublishersWiley Periodicals, Inc., 2000-10)
Details for predicting secondary structure of RNA sequences using free energy minimization are given. Protocols present the use of the computer program RNAstructure (for PCs) and the mfold server (for Unix platforms). The ...
DNA Synthesis Without Base Protection
(Wiley Periodicals, Inc.Columbia University Press, 2004-09)
DNA synthesis can be achieved by using O‐selective methods for internucleotide bond formation. This greatly simplifies the synthesis of oligodeoxyribonucleotides by eliminating the need for nucleobase protection and ...
Use of Fluorescence Spectroscopy to Elucidate RNA Folding Pathways
(Wiley Periodicals, Inc.W.H. Freeman and Company, 2002-06)
This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast ...
Probing RNA Structures with Hydroxyl Radicals
(Wiley Periodicals, Inc., 2000-02)
Fe(II)‐EDTA can be used to conveniently generate hydroxyl radicals to promote cleavage of RNA at nucleotide resolution. Two procedures are described, involving the generation of free radicals from solvated molecular oxygen ...
Thin‐Layer Chromatography
(Wiley Periodicals, Inc.CRC Press,, 2000-12)
TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal ...
Conjugation of 5′‐Functionalized Oligodeoxyribonucleotides with Properly Functionalized Ligands
(IRL PressWiley Periodicals, Inc., 2001-12)
This unit reports the synthesis of oligodeoxyribonucleotides covalently linked via their 5′ termini to various ligands such as intercalating agents, reactive groups, or labels. Methods for incorporation of halogenoalkyl, ...
Fluorinated α-aminophosphonates—a new type of irreversible inhibitors of serine hydrolases
(Nauka/Interperiodica; MAIK “Nauka/Interperiodica” ; Springer Science+Business Media, 2005-01)
Rapid Magnesium Chelation as a Method to Study Real‐Time Tertiary Unfolding of RNA
(Wiley Periodicals, Inc.MIT Press, 2001-09)
This unit describes a method to measure the unfolding of RNA tertiary structure on a millisecond time scale. A stopped‐flow spectrophotometer is used to measure the rate of unfolding induced by the addition of EDTA to an ...