Now showing items 121-130 of 165
Regioselective 2′‐Silylation of Purine Ribonucleosides for Phosphoramidite RNA Synthesis
(Wiley Periodicals, Inc., 2001-09)
This unit describes high‐yield procedures for protection of purine ribonucleosides based on a reaction that allows concomitant highly regioselective 2′‐silylation and 3′‐phosphitylation to give, in one step, monomers that ...
Time‐Resolved Hydroxyl Radical Footprinting of RNA with X‐Rays
(Wiley Periodicals, Inc.Practical Applications., 2001-09)
RNA tertiary structure and protein interactions can be detected by protection from hydroxyl radical cleavage. Generation of hydroxyl radicals with a synchrotron X‐ray beam provides analysis on a short timescale (50 msec ...
Use of Chemical Modification To Elucidate RNA Folding Pathways
(Wiley Periodicals, Inc., 2002-06)
As discussed in this overview, chemical modification is sensitive to the accessibility of a nucleotide to the solvent, and many nucleotides become less accessible as an RNA folds into its structured form. Chemical modification ...
Modification of the 5′ Terminus of Oligodeoxyribonucleotides for Conjugation with Ligands
(Wiley Periodicals, Inc.IRL Press, 2001-07)
Ligands can be introduced at the 5′ terminus of an oligonucleotide by adding a linker to the ligand and modifying the 5′ terminus of the oligonucleotide. These are then reacted to give the ligand‐oligonucleotide conjugate. ...
In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization
(Oxford University PressWiley Periodicals, Inc., 2000-02)
This unit describes the selection of aptamers from a pool of single‐stranded RNA by binding to a protein target. Aptamers generated from this selection experiment can potentially function as protein inhibitors, and may ...
Molecular Modeling of Nucleic Acid Structure: Setup and Analysis
(Wiley Periodicals, Inc.Oxford University Press, 2001-09)
The last in a set of units by these authors, this unit addresses some important remaining questions about molecular modeling of nucleic acids. It describes how to choose an appropriate molecular mechanics force field; how ...
Thermal Methods for the Analysis of RNA Folding Pathways
(John Wiley & Sons, 2000-10)
Once a model of the secondary structure of an RNA has been deduced, thermal melting analysis can be used to determine whether the model accounts for all intramolecular interactions of the RNA, or whether noncanonical and ...
Molecular Modeling of Nucleic Acid Structure: Electrostatics and Solvation
(Wiley Periodicals, Inc.Oxford University Press, 2001-07)
This unit presents an overview of computer simulation techniques as applied to nucleic acid systems, ranging from simple in vacuo molecular modeling techniques to more complete all‐atom molecular dynamics treatments that ...
Denaturing Polyacrylamide Gel Electrophoresis
(Wiley Periodicals, Inc., 2000-02)
Thin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (<500 nucleotides) single‐stranded fragments of DNA or RNA that differ in length by as little as one nucleotide. ...