Now showing items 141-150 of 165
Analyzing Radioligand Binding Data
(Raven Press,Wiley Periodicals, Inc., 2000-09)
A radioligand is a radioactively labeled drug that can associate with a receptor, transporter, enzyme, or any protein of interest. Measuring the rate and extent of binding provides information on the number of binding ...
Cartridge Methods for Oligonucleotide Purification
(Wiley Periodicals, Inc., 2000-07)
Protocols are given for purification of oligonucleotides by dimethoxytrityl‐sensitive and affinity desalting methods. The protocols are applicable for many of the convenient disposable products available for rapid ...
Chemistry of CpG DNA
(Wiley Periodicals, Inc., 2003-03)
The vertebrate immune system can recognize specific pathogen‐associated molecular patterns in invading microorganisms, including the unmethylated CpG dinucleotide. This unit discusses the receptors that recognize CpG motifs ...
Incorporation of Halogenoalkyl, 2‐Pyridyldithioalkyl, or Isothiocyanate Linkers into Ligands
(IRL PressWiley Periodicals, Inc., 2001-07)
Ligands can be introduced at the 5’ terminus of an oligonucleotide by adding a linker to the ligand and modifying the 5’ terminus of the oligonucleotide. These are then reacted to give the ligand‐oligonucleotide conjugate. ...
Raulin award lecture: Wilson's disease therapy with zinc and tetrathiomolybdate
(John Wiley & Sons, Inc., 2000)
Zinc and tetrathiomolybdate, effective, nontoxic drugs, have now been added to the treatment of most phases of Wilson's disease. Zinc is used for maintenance as well as treating presymptomatic, pregnant, and pediatric ...
Synthetic Strategies and Parameters Involved in the Synthesis of Oligodeoxyribo‐ and Oligoribonucleotides According to the H‐Phosphonate Method
(Wiley Periodicals, Inc.Humana Press, 2000-02)
Oligonucleotides can be synthesized by condensing a protected nucleoside H‐phosphonate monoester with a second nucleoside in the presence of a coupling agent to produce a dinucleoside H‐phosphonate diester. This can then ...
Analysis and Purification of Synthetic Nucleic Acids Using HPLC
(Wiley Periodicals, Inc.Stockton Press, 2000-07)
HPLC is a powerful and popular method for analyzing and purifying biomolecules. Reversed‐phase HPLC allows a high‐capacity method for purification, and uses volatile buffer systems that simplify product recovery. Anion‐exchange ...
A turn in the road: How studies on the pharmacology of glucosylceramide synthase inhibitors led to the identification of a lysosomal phospholipase A 2 with ceramide transacylase activity
(Kluwer Academic Publishers; Springer Science+Business Media, 2003-01)
A series of inhibitors of glucosylceramide synthesis, the PDMP based family of compounds, has been developed as a tool for the study of sphingolipid biochemistry and biology. During the course of developing more active ...
The 4â Methylthioâ 1â Butyl Group for Phosphate/Thiophosphate Protection in Oligodeoxyribonucleotide Synthesis
(Wiley Periodicals, Inc., 2004-12)
The detailed preparation of deoxyribonucleoside phosphoramidites functionalized with a 4â methylthioâ 1â butyl group for P(III) protection is described, along with the incorporation of these phosphoramidites into DNA ...
Commentary: Landmark articles on copper in the field of human health
(John Wiley & Sons, Inc., 2001)
No abstract.