Now showing items 151-160 of 165
Development of a Universal Nucleobase and Modified Nucleobases for Expanding the Genetic Code
(W.H. Freeman and Co.Wiley Periodicals, Inc., 2002-09)
This unit presents protocols for the synthesis and characterization of nucleosides with unnatural bases in order to develop bases for the expansion of the genetic alphabet or for nonselective pairing opposite natural bases. ...
Analysis of Oligonucleotides by Electrospray Ionization Mass Spectrometry
(Wiley Periodicals, Inc., 2000-02)
Because of the high molecular weights and thermal lability of biomolecules such as nucleic acids and protein, they can be difficult to analyze by mass spectrometry. Such analyses require a “soft” ionization method that is ...
Selection for Catalytic Function with Nucleic Acids
(Wiley Periodicals, Inc.Cold Spring Harbor, 2000-02)
For in vitro selection of catalytic polynucleotides, each new protocol must be designed to harness the desired catalytic activity to help propel the selection process itself. This unit gives guidelines for design of in ...
Attachment of Reporter and Conjugate Groups to the 3′ Termini of Oligonucleotides
(Wiley Periodicals, Inc.Humana Press, 2000-10)
Conjugation of oligonucleotides at the 3 terminus is less common because this site is used for covalent linkage to solid‐phase oligonucleotide synthesis supports. However, 3‐oligonucleotide conjugates have several valuable ...
Recent Advances in RNA Structure Determination by NMR
(John Wiley & Sons, 2000-10)
Despite recent advances in the solution of NMR structures of RNA and RNA‐ligand complexes, the rate limiting step remains the gathering of a large number of NOE and torsion restraints. Additional sources of information for ...
Analysis of Oxidized DNA Fragments by Gel Electrophoresis
(Wiley Periodicals, Inc., 2001-04)
Polyacrylamide gel electrophoresis is used to define and quantify products of deoxyribose oxidation in DNA, based on the unique electrophoretic mobility of DNA fragments possessing deoxyribose oxidation products on their ...
NMR Determination of Oligonucleotide Structure
(Oxford University PressWiley Periodicals, Inc., 2000-02)
This unit provides an overview of the use of NMR to determine oligonucleotide structure. It covers basic NMR spectral properties, acquisition of interproton distance restraints and torsion angle restraints, structure ...
Solid‐Phase Synthesis of Branched Oligonucleotides
(Wiley Periodicals, Inc.Academic Press, 2002-06)
Branched nucleic acids (bNAs) have been of particular interest since the discovery of RNA forks and lariats as intermediates of nuclear mRNA splicing, as well as multicopy, single‐stranded DNA (msDNA). Such molecules contain ...
Chemical and Enzymatic Methods for Preparing Circular Single‐Stranded DNAs
(CRC PressWiley Periodicals, Inc., 2000-02)
Small circular oligonucleotides can be used for diagnostic, therapeutic, and laboratory purposes. These systems have gained considerable attention in recent years because they form unusually strong and specific complexes ...
3′‐Modified Oligonucleotides and their Conjugates
(Wiley Periodicals, Inc.Pierce Chemical Company, 2000-12)
Solid‐phase synthesis of 3′‐aminoalkyl, 3′‐thioalkyl, and 3′‐polyethyleneglycol are described. The first two are important as specific points of attachment for a large variety of reporter groups; the latter is important ...