Now showing items 41-50 of 165
Design, Synthesis, and Amplification of DNA Pools for In Vitro Selection
(PerSeptive BiosystemsWiley Periodicals, Inc., 2000-02)
Preparation of a random‐sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer. Purification of a single‐stranded ...
Synthesis of Oligoribonucleotides Using the 2‐Nitrobenzyloxymethyl Group for 2′‐Hydroxyl Protection
(Wiley Periodicals, Inc., 2000-12)
RNA can be rapidly synthesized using protected ribonucleoside phosphoramidite monomers that carry 2‐nitrobenzyloxymethyl groups on their 2‐hydroxyls. These protecting groups are removed from the oligoribonucleotide products ...
Chemistry of Minor Groove Binder–Oligonucleotide Conjugates
(CRC PressWiley Periodicals, Inc., 2003-06)
Various types of minor groove binders have been attached to synthetic oligodeoxynucleotides, and the interactions of these conjugates (MB‐ODNs) with DNA are reviewed here. MB‐ODNs have enhanced DNA affinity and have improved ...
Engineering Disulfide Cross‐Links in RNA Using Thiol‐Disulfide Interchange Chemistry
(John Wiley & Sons, 2000-02)
Protocols for postsynthetic modification of 2‐amino‐containing oligoribonucleotides with either an alkyl‐phenyl disulfide or an alkyl thiol group are described. These groups react under mild conditions to form disulfide ...
Enzymatic Synthesis of M1Gâ Deoxyribose
(PlenumWiley Periodicals, Inc., 2000-02)
Adducts formed between electrophiles and nucleic acid bases are believed to play a key role in chemically induced mutations and cancer. M1Gâ dR is an endogenous exocyclic DNA adduct formed by the reaction of the dicarbonyl ...
Useful Nucleic Acid Chemistry Web Sites
(Wiley Periodicals, Inc., 2000-02)
This appendix provides a listing of selected internet resources found to be of particular value by the CPNC editorial board; it is not intended to be a complete listing of all web resources cited in this manual. In addition ...
Molecular Modeling of Nucleic Acid Structure
(Wiley Periodicals, Inc.Data commission of the international union of crystallography, 2001-09)
This unit is the first in a series of four units covering the analysis of nucleic acid structure by molecular modeling. This unit provides an overview of computer simulation of nucleic acids. Topics include the static ...
Deoxyribo‐ and Ribonucleoside H‐Phosphonates
(Humana PressWiley Periodicals, Inc., 2001-04)
Most methods for preparing H‐phosphonate monoesters suffer from variable yields and are often incompatible with common protecting groups used in oligonucleotide synthesis. This unit describes four procedures that consistently ...
Nucleobase Protection of Deoxyribo‐ and Ribonucleosides
(Wiley Periodicals, Inc.CRC Press, 2000-02)
Protecting groups for the imide/lactam function of thymine/uracil and guanine, respectively, prevent irreversible nucleobase modifications that may occur in the presence of alkylating or condensing reagents that are commonly ...
Optical Methods
(Elsevier Science Ltd.Wiley Periodicals, Inc., 2000-02)
Equilibrium optical melting curves are used in this unit to determine thermodynamic parameters of nucleic acid complex formation. This section contains all of the relevant equations and a discussion of which are most ...