Now showing items 61-70 of 165
Oligodeoxyribonucleotide Analogs Functionalized with Phosphonoacetate and Thiophosphonoacetate Diesters
(Humana PressWiley Periodicals, Inc., 2004-09)
Oligodeoxyribonucleotides with phosphonoacetate or thiophosphonoacetate internucleotide linkages can be made in high yield by solid‐phase synthesis and possess many advantages. They are highly stable to nucleases, ...
Theoretical Principles of In Vitro Selection Using Combinatorial Nucleic Acid Libraries
(Wiley Periodicals, Inc., 2000-02)
A new paradigm for drug discovery and biological research has developed from technologies that integrate combinatorial chemistry with rounds of selection and amplification, a technique called in vitro selection or systematic ...
Preparation of Gold Nanoparticle–DNA Conjugates
(Wiley Periodicals, Inc., 2002-06)
This unit describes the preparation of conjugates between nanometer‐scale gold particles and synthetic oligonucleotides. Oligonucleotide‐functionalized gold nanoparticles are finding increased use in both the construction ...
Derivatization of Glass and Polypropylene Surfaces
(Wiley Periodicals, Inc., 2004-09)
This unit describes the derivatization of solid support media, particularly glass and polypropylene, with a linker system by an iterative process that forms a dendrimeric structure, thereby increasing stepwise the loading ...
Synthesis and Characterization of Chimeric 2‐5A‐DNA Oligonucleotides
(Wiley Periodicals, Inc.Elsevier/ North‐Holland, 2000-07)
This unit provides protocols for the synthesis and characterization of 2‐5A‐antisense nucleic acids. These chimeric oligonucleotides consist of 2′,5′‐phosphodiester‐linked oligoadenylates ligated to 3′,5′‐deoxyribonucleotides ...
Proteomic Analysis Using 2‐D Liquid Separations of Intact Proteins From Whole‐Cell Lysates
(Marcel DekkerWiley Periodicals, Inc., 2003-11)
This unit describes procedures for 2‐D liquid separations of proteins from whole‐cell lysates. Protocols for protein isoelectric point (pI) fractionation in the first dimension include the use of liquid isoelectric focusing ...