Now showing items 11-19 of 19
Observations consistent with autocrine stimulation of hybridoma cell growth and implications for large-scale antibody production
(Kluwer Academic Publishers; Springer Science+Business Media, 1992-04)
Existence of autocrine growth factors (aGFs) may influence the serum requirement for growth of hybridoma cells and thus significantly influence process economics. For the murine hybridoma cell line S3H5/γ2bA2, critical ...
[alpha]-(2 --> 3)- and [alpha]-(2 --> 6)-Sialyltransferase activities present in three variants of Ehrlich tumor cells: identification of the products derived from N-acetyllactosamine and [beta]--Gal-(1 --> 3)-[alpha] GalNAc-(1 --> O)-Bn
(Elsevier, 1994-11-01)
We compared several sialyltransferase activities related to synthesis of O-linked and N-linked sialylglycoproteins in Ehrlich ascites tumor cells that grow normally in murine ascites, but are not adherent nor grow in tissue ...
Control of gene expression at the level of translation initiation
(Elsevier, 1994-10)
Protein synthesis is controlled at the level of translation initiation. Cells rapidly respond to environmental changes by disassembly of polysomes and recruitment of specific mRNAs from inactive ribonucleoprotein particles ...
Effect of calcium chloride treatment on hybridoma cell viability and growth
(Kluwer Academic Publishers; Springer Science+Business Media, 1992-10)
In order to minimize hybridoma cell damage during calcium alginate entrapment, the effect of calcium chloride treatment on hybridoma cell viability and growth was studied in terms of calcium chloride concentration and ...
Simplified method of making alginate-polylysine microcapsules for hybridoma cell culture using RPMI 1640 medium
(Kluwer Academic Publishers; Science & Technology Letters ; Springer Science+Business Media, 1990-09)
The method of making alginate-poly-L-lysine microcapsules for hybridoma cell culture can be simplified by cultivating the cells in RPMI 1640 medium. Phosphate concentration in RPMI 1640 medium is sufficiently high to ...
Effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridomas during batch culture
(Kluwer Academic Publishers; Science & Technology Letters ; Springer Science+Business Media, 1993-04)
In order to investigate the effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridoma cells, cells in different growth stages during a batch culture were fixed and stored ...