Now showing items 51-60 of 692
New vectors for high level expression of recombinant proteins in bacteria
(Elsevier, 1992-05-01)
A system has been developed for synthesis and rapid purification of recombinant polypeptides expressed in frame with glutathione S-transferase ([3.], Gene 67, 31-40). Expressed fusion proteins are purified from bacterial ...
Fluorescence Yield Near Edge Spectroscopy (FYNES) of benzene adsorbed in Na-Y zeolite
(Baltzer Science Publishers, Baarn/Kluwer Academic Publishers; J.C. Baltzer AG, Scientific Publishing Company ; Springer Science+Business Media, 1990-01)
Benzene adsorbed in sodium-Y zeolite has been investigated using Fluorescence Yield Near Edge Spectroscopy (FYNES). These studies demonstrate that FYNES measurements are feasible as a direct probe of the local electronic ...
Structural organization of the bovine gene for the heart/muscle isoform of cytochrome c oxidase subunit VIa
(Elsevier, 1993-07-18)
The bovine gene for the nuclear-encoded heart/muscle isoform of cytochrome c oxidase subunit VIa (COX6A1) was isolated from a library of bovine genomic DNA in lambda EMBL3 and sequenced. The gene spans 760 bp and comprises ...
Determination of the electro-optical coefficient r33 and refractive index of para-substituted poly([gamma]-benzyl--glutamate) derivatives
(Elsevier, 1994-01)
The electro-optical coefficient r33 and refractive index of films of poly([gamma]-benzyl--glutamate) and the p-nitro, p-fluoro and p-trifluoromethyl derivatives were determined as a way to assess the non-linear optical ...
Mechanism and pharmacological specificity of dUTPase-mediated protection from DNA damage and cytotoxicity in human tumor cells
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1998-09)
Purpose : We have reported previously that the expression of E. coli dUTPase (dutE) can protect HT29 cells from 5-fluorodeoxyuridine (FdUrd)-induced DNA fragmentation and cytotoxicity. In the study reported here, we further ...
Biological effects of a novel intestinal peptide -- inhibiting enterocytogenin on cultured 3T3 mouse fibroblasts and L5178Y mouse lymphoma cells
(Elsevier, 1994-05-05)
The effects of a new intestinal peptide, inhibiting enterocytogenin (IEG) derived from pig intestinal mucosa were studied in vitro on 3T3 mouse fibroblasts and L5178Y mouse lymphoma cell line. IEG caused considerable growth ...
An intramolecular, Schmidt reaction of an alkyl azide with a carbocation. The generation and rearrangement of a conformationally restricted secondary aminodiazonium ion.
(Elsevier, 1992-09-08)
Treatment of azidoalkene 2 with triflic acid produced the bridged bicyclic enamine 3 by an intramolecular Schmidt reaction. Rearrangement of the intermediate aminodiazonium ion 5 is completely regioselective.
High-resolution analysis of c-fos chromatin accessibility using a novel DNase I-PCR assay
(Elsevier, 1992-04-06)
In our previous study, c-fos chromatin accessibility was assayed using DNase I digestion and Southern blot analysis. This low-resolution mapping of c-fos chromatin accessibility demonstrated that serum stimulation of the ...