Now showing items 31-40 of 70
Purification and Characterization of a Mannose-Specific Lectin from Shallot (Allium ascalonicum) Bulbs
(Elsevier, 1993-11)
A new mannose-binding lectin was isolated from shallot (Allium ascalonicum) bulbs by affinity chromatography on an immobilized D-mannose column. The lectin (A. ascojonicum agglutinin, AAA) appeared homogeneous by polyacrylamide ...
Cultured Ehrlich Ascites Tumor Cells Show Increased N-Linked [alpha]2,6-Sialytransferase Activity
(Elsevier, 1993-06)
Ehrlich ascites tumor cells (EAT cells) are routinely grown in the peritoneal cavity of mice. These cells, EAT-wt, grow in suspension and exhibit a high level of [alpha]-2,3-O-linked sialyltransferase activity with ...
Localization of the ~12 kDa Mr discrepancy in gel migration of the mouse glucocorticoid receptor to the major phosphorylated cyanogen bromide fragment in the transactivating domain
(Elsevier, 1993-12)
The intact wild-type mouse glucocorticoid receptor has a theoretical molecular weight of ~86 kDa based on amino acid sequence, but on SDS-polyacrylamide gel electrophoresis it migrates as a protein of ~98 kDa. It is not ...
The hsp56 immunophilin component of steroid receptor heterocomplexes: Could this be the elusive nuclear localization signal-binding protein?
(Elsevier, 1993-09)
In many cells, the glucocorticoid receptor undergoes rapid steroid-mediated translocation from the cytoplasm to the nucleus, and this receptor is an excellent model for studying the mechanism of targeted protein movement ...
Determination of plasma propofol levels using gas chromatography--mass spectrometry with selected-ion monitoring
(Elsevier, 1993-10-29)
Propofol (2,6-diisopropylphenol, I.C.I. 35 868) is a rapid-acting, intravenous anesthetic agent recently introduced for the induction and maintenance of general anesthesia. This paper describes a gas chromatographic--mass ...
Baculovirus-Mediated Expression of Rat Liver CTP:Phosphocholine Cytidylyltransferase
(Elsevier, 1993-02)
We describe herein the expression and purification of milligram quantities of rat liver CTP:phosphocholine cytidylyltransferase in recombinant baculovirus-infected insect cells. The enzyme was purified by incorporating ...
Inhibition of calcification of glutaraldehyde pretreated porcine aortic valve cusps with sodium dodecyl sulfate: Preincubation and controlled release studies
(John Wiley & Sons, Inc., 1993-12)
Calcification of bioprosthetic heart valves fabricated from glutaraldehyde pretreated bovine pericardium or porcine aortic valves (PAV) is a frequent cause of the failure of these devices. Of all strategies considered thus ...
Model studies of lantibiotic biogenesis
(Elsevier, 1993-12-03)
A six residue polypeptide has been synthesized to study the proposed biosynthesis of lanthionine. It is shown that this peptide spontaneously cyclizes in a biomimetic fashion via a completely stereoselective intramolecular ...