Now showing items 61-70 of 104
A computational approach to inferring cellular protein-binding affinities from quantitative fluorescence resonance energy transfer imaging
(WILEY-VCH Verlag, 2009-12)
Fluorescence resonance energy transfer (FRET) microscopy can measure the spatial distribution of protein interactions inside live cells. Such experiments give rise to complex data sets with many images of single cells, ...
Toward high sequence coverage of proteins in human breast cancer cells using on-line monolith-based HPLC-ESI-TOF MS compared to CE MS
(WILEY-VCH Verlag, 2006-06)
A method is developed toward high sequence coverage of proteins isolated from human breast cancer MCF10 cell lines using a 2-D liquid separations. Monolithic-capillary columns prepared by copolymerizing styrene with ...
CE of poly(amidoamine) succinamic acid dendrimers using a poly(vinyl alcohol)-coated capillary
(WILEY-VCH Verlag, 2008-01)
Various generations (G1–G8) of negatively charged poly(amidoamine) (PAMAM) succinamic acid dendrimers (PAMAM-SAH) were analyzed by CE using a poly(vinyl alcohol)-coated capillary. Due to its excellent stability and osmotic ...
Electrophoretic mobility and molecular distribution studies of poly(amidoamine) dendrimers of defined charges
(WILEY-VCH Verlag, 2006-05)
Generation 5 ethylenediamine (EDA)-cored poly(amidoamine) (PAMAM) dendrimers (E5, E denotes the EDA core and 5 the generation number) with different degrees of acetylation and carboxylation were synthesized and used as a ...
BiomarkerDigger: A versatile disease proteome database and analysis platform for the identification of plasma cancer biomarkers
(WILEY-VCH Verlag, 2009-07)
We have developed a proteome database (DB), BiomarkerDigger ( http://biomarkerdigger.org ) that automates data analysis, searching, and metadata-gathering function. The metadata-gathering function searches proteome DBs for ...
A comparative phosphoproteomic analysis of a human tumor metastasis model using a label-free quantitative approach
(WILEY-VCH Verlag, 2010-06)
Alterations in cellular phosphorylation patterns have been implicated in a number of diseases, including cancer, through multiple mechanisms. Herein we present a survey of the phosphorylation profiles of an isogenic pair ...
Validation of reverse phase protein array for practical screening of potential biomarkers in serum and plasma: Accurate detection of CA19-9 levels in pancreatic cancer
(WILEY-VCH Verlag, 2008-08)
The current study analyzed reverse phase protein arrays (RPPA) as a means to experimentally validate biomarkers in blood samples. One microliter samples of sera ( n 14= 1471), and plasma ( n 14= 1478) were serially diluted ...
Capillary electrophoresis–electrochemiluminescence detection method for the analysis of ibandronate in drug formulations and human urine
(WILEY‐VCH Verlag, 2011-08)
A simple, rapid and sensitive CE method coupled with electrochemiluminescence (ECL) detection for direct analysis of ibandronate (IBAN) has been developed. Using a buffer solution of 20 mM sodium phosphate (pH 9.0) and a ...
The analysis of alphaâ 1â antitrypsin glycosylation with direct LCâ MS/MS
(Cold Spring Harbor Laboratory PressWiley Periodicals, Inc., 2018-09)
A liquid chromatographyâ tandem mass spectrometry (LCâ MS/MS)â based methodology has been developed to differentiate coreâ and antennaryâ fucosylated glycosylation of glycopeptides. Both the glycosylation sites ...