Now showing items 61-70 of 809
Benzimidazole ribonucleosides: Observation of an unexpected nitration when performing non-aqueous diazotizations with t-butyl nitrite
(Elsevier, 1992-09)
Mild, non-acidic conditions used in the non-aqueous diazotization of a 2-aminobenzimidazole nucleoside with t-butyl nitrite gave an unexpected 4-nitrobenzimidazol-2-one nucleoside which was not formed when primary alkyl ...
A sensitive coupled HPLC/RIA technique for separation of endorphins: Multiple forms of B-endorphin in rat pituitary intermediate vs. anterior lobe
(Elsevier, 1981-08)
A technique is described which couples High Performance Liquid Chromatography (HPLC) to radioimmuno assays (RIA), thus achieving high sensitivity with good specificity. This method allows the separation and detection of ...
Base Composition Analysis of Nucleosides Using HPLC
(Applied Biosystems,Wiley Periodicals, Inc., 2000-07)
In this protocol, nuclease digestion of an oligonucleotide is followed by dephosphorylation and HPLC analysis of the monomers on a reversed‐phase C18 column. This method can be used to detect and quantitate a wide variety ...
A method to reduce interference by sucrose in the detection of thiobarbituric acid-reactive substances
(Elsevier, 1984-03)
A thiobarbituric acid (TBA) reaction for measuring lipid peroxidation products was evaluated for interference by several ingredients commonly used in solutions to prepare or analyze tissue homogenates or subcellular ...
Hydrogenation of testosterone sulfate by the [Delta]4-3-ketosteroid reductase of rat liver microsomes
(Elsevier, 1965-01)
TPNH2 is oxidized in the presence of testosterone or testosterone sulfate and a microsomal preparation from the liver of female rats. In the presence of iorganic phosphate, DPNH2 also acts as a hydrogen donor. Evidence is ...
Aspirin as a quantitative acetylating reagent for the fatty acid oxygenase that forms prostaglandins
(Elsevier, 1976-01)
A selective acetylation of the prostaglandin-forming fatty acid oxygenase (part of the prostaglandin "synthetase" system) occurs with 100 [mu]M concentrations of aspirin (acetylsalicylic acid). The amount of acetylation, ...
New vectors for high level expression of recombinant proteins in bacteria
(Elsevier, 1992-05-01)
A system has been developed for synthesis and rapid purification of recombinant polypeptides expressed in frame with glutathione S-transferase ([3.], Gene 67, 31-40). Expressed fusion proteins are purified from bacterial ...
Differences in hydroxysTEROL METABOLISM BETWEEN RAT AND MOUSE LEYDIG CELLS
(Elsevier, 1984-06)
Electroencephalographic assessment of the role of [delta] receptors in opioid peptide - induced seizures
(Elsevier, 1984-12)
DPDPE ([D-Pen2, D-Pen5]-Enkephalin) and DPLPE ([D-Pen2, L-Pen5]-Enkephalin) are conformationally-constrained cyclic analogs of enkephalin with high selectivity for [delta] opioid receptors. Intracerebroventricular (i.c.v.) ...