Now showing items 21-30 of 809
The cluster-tray method for rapid measurement of solute fluxes in adherent cultured cells
(Elsevier, 1981-08)
The use of an inexpensive and simple modification of Costar 24-well cluster trays is described in a rapid and reproducible method for measuring substrate fluxes in adherent cultured eukaryotic cells.
CCK antagonists reveal that CCK-8 and JMV-180 interact with different sites on the rat pancreatic acinar cell CCKA receptor
(Elsevier, 1994)
The ability of CCKA antagonists to inhibit full and partial CCK agonists of the rat pancreatic acinar cell CCKA receptor has been studied. When isolated rat pancreatic acini were superfused with CCK-8 (10 pM-1 nM) or CCK-4 ...
The use of silica gel impregnated glass fiber sheets in plasma estriol assays
(Elsevier, 1970)
The fluorimetric quantitation of nanogram amounts of estriol extracted from plasma is sometimes impaired by impurities which interfere with fluorescence. Instant thin-layer chromatography on silica gel impregnated glass ...
Engineering Specific Cross‐Links in Nucleic Acids Using Glycol Linkers
(Wiley Periodicals, Inc., 2000-02)
One of the most convenient methods for generating oligonucleotides possessing intra‐ or interstrand cross‐links is through incorporation of oligoethylene glycol bridges by solid‐phase synthesis. The reagents are commercially ...
A polarographic method for following the rates of cholinesterase-catalyzed hydrolyses of acetylthiocholine
(Elsevier, 1965-08)
A rapid and simple polarographic method for following the rates of the cholinesterase-catalyzed hydrolysis of acetylthiocholine and the rates of inhibition of this hydrolysis is described. This method has the advantage ...
Key Experimental Approaches in DNA Nanotechnology
(Alan R. LissWiley Periodicals, Inc., 2002-06)
DNA nanotechnology combines unusual DNA motifs with sticky‐ended cohesion to build polyhedral objects, topological targets, nanomechanical devices, and both crystalline and aperiodic arrays. The goal of DNA nanotechnology ...
Probing RNA Structure with Chemical Reagents and Enzymes
(Wiley Periodicals, Inc., 2000-02)
This unit provides thorough coverage of the most useful chemical and enzyme probes that can be used to examine RNA secondary and tertiary structure. Footprinting methods are presented using dimethyl sulfate, diethyl ...
cDNA and derived amino acid sequence of rabbit nasal cytochrome P450NMb (P450IIG1), a unique isozyme possibly involved in olfaction
(Elsevier, 1991-02-15)
Olfactory-specific cytochrome P450NMb was previously purified to electrophoretic homogeneity from microsomes of rabbit nasal mucosa in this laboratory. In the present study, a cDNA library made from poly(A)+ RNA from rabbit ...