Now showing items 791-800 of 809
Reconstitution of oxidative phosphorylation in submitochondrial particles by a soluble protein phosphoryl transferase
(Elsevier, 1968-01)
A protein designated phosphoryl transferase has been isolated from beef heart mitochondria and has been found to increase the ability of Submitochondrial particles, oxidizing either NADH or succinate, to synthesize ATP ...
Probing Nucleic Acid Structure with Nickel‐ and Cobalt‐Based Reagents
(Kluwer Academic PublishersWiley Periodicals, Inc., 2000-02)
The use of nickel and cobalt reagents is presented for characterizing the solvent exposure of guanine residues in DNA and RNA. These reagents promote guanine oxidation in the presence of a peracid such as monopersulfate, ...
Gas chromatographic determination of gallopamil and norgallopamil in human plasma
(Elsevier, 1989)
A highly sensitive gas chromatographic assay is described for the simultaneous determination of gallopamil, a calcium channel blocking agent, and its major metabolite, norgallopamil. A multi-step extraction procedure is ...
The effect of daily ingestion of caffeine on the microsomal enzymes of rat liver
(Elsevier, 1973-02)
The effect of repeated ingestion of caffeine on rat-liver microsoma enzymes was studied. In the rat, ingestion of 20-24 mg caffeine/kg/day in the drinking water for more than 2 wk inhibited aminopyrine N-demethylase activity. ...
Iodine analysis of biological samples by neutron activation of 127I, with scintillation counting of cerenkov radiation
(Elsevier, 1971-03)
The naturally occurring isotope, 127I, has been measured by neutron activation, postirradiation extraction of 128I plus 127I (added carrier), and timed scintillation counting of aqueous solution utilizing Cerenkov radiation. ...
Measures of viability in isolated cells
(Elsevier, 1975-06)
Various methods were compared for estimation of cell viability. Cell count, uptake of trypan blue, release of cellular LDH, as well as cell contents of K+ and Na+ were determined in leukocytes, spleen cells, and Ehrlich ...
Engineering Disulfide Cross‐Links in RNA Via Air Oxidation
(Oxford University PressWiley Periodicals, Inc., 2000-02)
This unit presents protocols for the synthesis of alkylthiol‐modified ribonucleosides, their incorporation into synthetic RNA, and the formation of intramolecular disulfide bonds in RNA by air oxidation. The disulfide bonds ...
Assay and properties of the enzyme catalyzing the biosynthesis of 1-O-alkyl dihydroxyacetone 3-phosphate
(Elsevier, 1981-10-01)
The properties of the enzyme alkyl dihydroxyacetone phosphate synthase (1-O-acyl dihydroxyacetone 3-phosphate + ROH --> 1-O-alkyl dihydroxyacetone 3-phosphate + fatty acid) in Ehrlich ascites tumor cell microsomes were ...
Introduction to the Synthesis and Purification of Oligonucleotides
(Applied BiosystemsWiley Periodicals, Inc., 2000-02)
Modern nucleic acid synthesizers utilize phosphite triester chemistries that employ stable phosphoramidite monomers to build a growing polymer. These robust reactions allow easy generation of specific oligodeoxyribo‐ and ...