Now showing items 931-940 of 945
Quantitative in vivo receptor binding IV: Detection of muscarinic receptor down-regulation by equilibrium and by tracer kinetic methods
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1991-09)
Newly-developed methods for estimation of in vivo binding to neurotransmitter receptors should enable the detection and quantification of physiologic or pathologic changes in receptor numbers. In the present study, both ...
Affinity of the antiviral enantiomers of oxathiolane cytosine nucleosides for human 2'-deoxycytidine kinase
(Elsevier, 1993-04-06)
The two enantiomers of 2',3'-dideoxy-3'-thiacytidine (BCH-189) and their 5-fluoro analogs (FTC) were found to be good substrates for human 2'-deoxycytidine kinase with Km values in the 5.7 to 42.1 [mu]M range. The affinity ...
Formation of a toxic metabolite from gentamicin by a hepatic cytosolic fraction
(Elsevier, 1992-04-15)
We have demonstrated recently that incubation of the aminoglycoside gentamicin with an hepatic post-mitochondrial fraction produces a compound toxic to sensory cells from the inner ear in short-term culture; in contrast, ...
Myoglobin models and steric origins of the discrimination between O 2 and CO
(Springer-Verlag; Society of Biological Inorganic Chemistry, 1997-08)
Synthetic models of the myoglobin active site have provided much insight into factors that affect CO and O 2 binding in the proteins. "Capped" and "pocket" metal porphyrin systems have been developed to probe how steric ...
Specific steroid response from a nonspecific DNA element
(Elsevier, 1994-06)
A fundamental dilemma of steroid hormone regulation is how specific transcription is attained in vivo when several receptors recognize the same DNA sequence in vitro. We have identified an enhancer of the mouse sex-limited ...
Dinucleotide polymorphism at the DXS1178 locus is tightly linked to PGK1 at Xq13
(Springer-Verlag, 1995-04)
A polymorphic CA repeat (locus name DXS1178 ) was isolated from a 1-megabase YAC (OTCC) containing the OTC gene, located at Xp21.1. However, amplification in human-rodent hybrid cells and segregation analysis in three CEPH ...
Enzymatic synthesis of sialyl Lex and derivatives based on a recombinant fucosyltransferase
(Elsevier, 1991)
A recombinant human Lewis [alpha](1,3/1,4)fucosyltranferase has been studied for its acceptor substrate specificity and used in the synthesis of sialyl Lex and derivatives.
Mass transfer effects on microbial uptake of naphthalene from complex NAPLs
(Wiley Subscription Services, Inc., A Wiley Company, 1998-12-20)
The bioavailability of naphthalene present as a component of a complex nonaqueous phase liquid (NAPL) comprised by nine aromatic compounds was investigated. Specifically, the effects of naphthalene mass transfer from the ...
Effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridomas during batch culture
(Kluwer Academic Publishers; Science & Technology Letters ; Springer Science+Business Media, 1993-04)
In order to investigate the effect of fixation temperature on flow cytometric measurement of intracellular antibody content of hybridoma cells, cells in different growth stages during a batch culture were fixed and stored ...
Adrenal proteins bound by a reactive intermediate of mitotane
(Springer-Verlag; Springer-Verlag Berlin Heidelberg, 1997-02)
Purpose : Mitotane ( o,p ′-DDD), is the only adrenolytic agent available for the treatment of adrenocortical carcinoma. Previous studies have shown that mitotane covalently binds to adrenal proteins following its metabolism ...