Now showing items 21-30 of 249
Protection of 2′‐Hydroxy Functions of Ribonucleosides
(Oxford University PressWiley Periodicals, Inc., 2000-02)
The main purpose of this article is to discuss 2′‐protection in the context of effective oligoribonucleotide synthesis. Emphasis is placed on the 2′‐protecting groups of choice in the synthesis of oligo‐and polyribonucleotides, ...
Comparative proteomic study of two closely related ovarian endometrioid adenocarcinoma cell lines using cIEF fractionation and pathway analysis
(WILEY-VCH Verlag, 2009-04)
The proteomic profiles from two distinct ovarian endometrioid tumor-derived cell lines, (MDAH-2774 and TOV-112D) each with different morphological characteristics and genetic mutations, have been studied. Characterization ...
Synthesis and Purification of Oligonucleotide N3′→︀P5′ Phosphoramidates and their Phosphodiester and Phosphorothioate Chimeras
(Wiley Periodicals, Inc., 2000-12)
This unit describes the synthesis and purification of oligonucleotide N3′→︀P5′ phosphoramidates, wherein each 3′‐oxygen is replaced by a 3′‐amine in the 2′‐deoxyribose ring. The synthesis of required monomers and application ...
Synthesis of the Tetraketide Lactones from the Pikromycin Biosynthetic Pathway
(WILEY-VCH Verlag, 2007-07)
Synthesis of tetraketide lactones 2 and 3 , which are likely to be produced by a model pikromycin polyketide synthase (PKS), has been investigated. The tetraketide lactones with six-membered rings, 2a and 2b , were ...
Site‐Specific Fluorescent Labeling of Large RNAs with Pyrene
(Oxford University PressWiley Periodicals, Inc., 2004-12)
Pyrene is a useful chromophore for monitoring the tertiary structure and folding of large RNAs. This unit describes the general preparation of a large RNA (>80 nucleotides in length) that has been site‐specifically modified ...
Preparation of 2′‐O‐[(Triisopropylsilyl)oxy]methyl‐protected Ribonucleosides
(Oxford University PressWiley Periodicals, Inc., 2001-12)
The [(triisopropylsilyl)oxy]methyl (TOM) group is a useful protecting group for the 2′‐OH of ribonucleosides to be used for oligoribonucleotide synthesis by the phosphoramidite method. It is completely stable to all reaction ...
A study of a C Α, Β -didehydroalanine homo-oligopeptide series in the solid-state by 13 C cross-polarization magic angle spinning NMR
(John Wiley & Sons, Ltd., 2004-06)
The fully extended peptide conformation (2.0 5 -helix) has been investigated for the first time in the solid-state by 13 C cross-polarization magic angle spinning NMR. The compounds examined are members of a terminally ...
Synthesis of Phosphorothioate Oligonucleotides with Stereodefined Phosphorothioate Linkages
(Wiley‐Liss, 2003-09)
A method for solid‐phase synthesis of stereodefined PS‐oligos via an oxathiaphospholane approach using pure P‐diastereomers of nucleoside oxathiaphospholane monomers is described. The oxathiaphospholane monomers are ...