Now showing items 31-40 of 148
Human PCK1 Encoding Phosphoenolpyruvate Carboxykinase Is Located on Chromosome 20q13.2
(Elsevier, 1993-01)
Cytoplasmic liver phosphoenolpyruvate carboxykinase (GTP)(PEPCK) catalyzes a rate-limiting step in gluconeogenesis. Primers derived from the rat liver PEPCK sequence were used to amplify a portion of the human liver cDNA ...
Genetic and Physical Map of 11 Short Tandem Repeat Polymorphisms on Human Chromosome 6
(Elsevier, 1993-01)
A linkage map of 11 short tandem repeat polymorphisms was constructed for human chromosome 6. The order from p to qter was F131-D6S105-D6S254-D6S251-D6S252-D6S249-(ARG1-D6S87)-D6S250-D6S255-D6S253. For the region spanned ...
Phosphoenolpyruvate Carboxykinase (GTP): Characterization of the Human PCK1 Gene and Localization Distal to MODY on Chromosome 20
(Elsevier, 1993-06)
The human PCK1 gene encoding phosphoenolpyruvate carboxykinase (GTP) (PEPCK) was isolated and sequenced. There is 91% amino acid sequence identity (567/622 residues) between the human and the rat proteins, with conservation ...
Loss of antibody productivity is highly reproducible in multiple hybridoma subclones
(Wiley Subscription Services, Inc., A Wiley Company, 1993-06-20)
An immunoglobulin G (IgG 2b ) producing hybridoma cell line (S3H5/Γ2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum-free medium. Following adaptation ...
Lactate Oxidation for the Detection of Mitochondrial Dysfunction in Human Skin Fibroblasts
(Elsevier, 1993-05-01)
To screen fibroblasts for defects in lactate/pyruvate oxidation, cells were grown to confluence in 25-cm2 flasks, rinsed, and incubated in glucose-free media containing 25 [mu]M L-lactate and 0.1 [mu]Ci [D,L-1-14C]lactate. ...
Infectivity of a glucan synthesis-defective mutant of Streptococcus gordonii (Challis) in a rat endocarditis model
(Elsevier, 1993-09-15)
Streptococcus gordonii, a member of the human indigenous oral microflora, colonizes smooth tooth surfaces and contributes to dental plaque formation. Although it is not recognized as being a cariogenic pathogen, it may ...
Thermodynamics of divalent-metal fluorides VI. Heat capacity and thermodynamic properties of the fast-ion conductor CaSn2F6 at temperatures from 6 K to 310 K
(Elsevier, 1993-02)
The molar heat capacity Cp. m of calcium hexafluorostannate CaSn2F6 was measured at temperatures T from 6 K to 310 K by adiabatic calorimetry. Reproducible heat capacities were obtained only after cooling the sample twice ...
Synthesis of 1-(2-aminopropyl)benzimidazoles, structurally related to the TIBO derivative R82150, with activity against human immunodeficiency virus.
(Elsevier, 1993-04)
A number of 1-(2-aminopropyl)-2-mercaptobenzimidazoles related to the TIBO derivatives R82150 have been prepared and tested for their activity against human immunodeficiency virus type 1 (HIV-1). These compounds were all ...