Now showing items 91-100 of 336
Protooncogene-encoded protein kinases in interleukin-2 signal transduction
(Elsevier, 1993-10)
Protooncogenes are the normal forms of cellular genes that when altered in their expression or coding sequences can contribute to neoplastic transformation. As these genes often are important for normal cellular growth ...
Decreased formation of inositol trisphosphate in Madin-Darby canine kidney cells under conditions of [beta]-glucosidase inhibition
(Elsevier, 1992-02-01)
Recent work has demonstrated the enhancement of hormone-stimulated inositol trisphosphate formation in renal epithelial cells under conditions of glucosylceramide depletion. The role of glucosylceramide metabolism was ...
1-Monoglyceride production from lipase-catalyzed esterification of glycerol and fatty acid in reverse micelles
(Wiley Subscription Services, Inc., A Wiley Company, 1991-08-20)
Glycerol-fatty acid esterification has been conducted with lipase from R. delemar in water/AOT/isooctane reverse micellar media, with the major product being 1-monoglyceride, a useful food-emulsifier. 1,3-diglyceride was ...
Comparison of the isolation of adducts fo 2'-deoxycytidine and 2'-deoxyguanosine with phenylglycidyl ether by high-performance liquid chromatography on a reversed-phase column and a polystyrene-divinylbenzene column
(Elsevier, 1991)
2'-Deoxycitidine (dCyd) and 2'-deoxyguanosine (dGuo) were subjected to reaction with phenylglycidyl ether (PGE) in methanol in order to study the formation of the corresponding 2'-deoxynucleoside adducts. Separation methods ...
Positive-ion thermospray liquid chromatography--mass spectrometry: detection of organic acidurias
(Elsevier, 1990)
Positive-ion thermospray liquid chromatography--mass spectrometry (TSP-LC-MS) is used to detect organic acids via the direct injection of untreated urine from newborns and infants. Two methods are reported for the separation ...
Frequent harvesting from perfused bone marrow cultures results in increased overall cell and progenitor expansion
(Wiley Subscription Services, Inc., A Wiley Company, 1994-08-20)
The establishment of prolific long-term human bone marrow cultures has led to the development of hematopoietic bioreactor systems. A single batch expansion of bone marrow mononuclear cell populations leads to a 10- to ...
Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production
(Wiley Subscription Services, Inc., A Wiley Company, 1992-02-20)
The influence of ammonia and lactate on cell growth, metabolic, and antibody production rates was investigated for murine hybridoma cell line 163.4G5.3 during batch culture. The specific growth rate was reduced by one-half ...
Microencapsulated human bone marrow cultures: A potential culture system for the clonal outgrowth of hematopoietic progenitor cells
(Wiley Subscription Services, Inc., A Wiley Company, 1994-04-05)
Currently the most successful methods for culturing human hematopoietic cells employ some form of perfused bioreactor system. However, these systems do not permit the clonal outgrowth of single progenitor cells. Therefore, ...
Ion-pair chromatography of inositol polyphosphates with N-methylimipramine
(Elsevier, 1990)
A novel counter-ion, N-methylimipramine, was synthesized and utilized in the separation of inositol phosphates by ion-pair chromatography. The structural identity of the counter-ion was documented by nuclear magnetic ...