Now showing items 751-760 of 812
Development of a Universal Nucleobase and Modified Nucleobases for Expanding the Genetic Code
(W.H. Freeman and Co.Wiley Periodicals, Inc., 2002-09)
This unit presents protocols for the synthesis and characterization of nucleosides with unnatural bases in order to develop bases for the expansion of the genetic alphabet or for nonselective pairing opposite natural bases. ...
Purification and properties of trehalase(s) from Neurospora
(Elsevier, 1963-09)
Trehalase has been obtained in crystalline form from the mycelium of Neurospora crassa. During elution from the first pass through a DEAE-cellulose column, two peaks with trehalase activity were obtained. When the first ...
Hadamard transform photothermal deflection desitometry of electrophretically blotted proteins
(Elsevier, 1990)
Hadamard transform spatial multiplexing techniques are applied to laser densitometry in order to prevent the photo-induced degradation of sensitive materials. Photochemical and thermal degradations can often occur in point ...
α- d -Galactosylation of surface fucoglycoconjugate(s) upon stimulation/activation of murine peritoneal macrophages
(Kluwer Academic Publishers; Chapman & Hall ; Springer Science+Business Media, 1992-04)
Murine resident macrophages express, on their surface, carbohydrate epitopes which undergo changes during their stimulation/activation as monitored by binding of 125 I labelled Evonymus europaea and Griffonia simplicifolia ...
Studies of the electron transport systems of heart muscle: Biochemical and antigenic properties of a soluble DPNH dehydrogenase
(Elsevier, 1968-04)
Highly purified DPNH dehydrogenase has been prepared from preparations of DPNH oxidase. The enzyme has an average molecular weight of 80,000 and contains flavin, nonheme iron, and labile sulfide groups in an approximate ...
Cholesterol biosynthesis in human fetal liver and adrenal
(Elsevier, 1971-07)
Hepatic and adrenal cholesterol biosynthesis were investigated in the human fetus in vitro by measuring the incorporation of acetate-2-14C into cholesterol by slices of liver and adrenals from the same fetus. To differentiate ...
Two cases of changes in amino acid transport activity with tissue development
(Elsevier, 1965)
During the first day of extrauterine life the liver of the guinea-pig greatly intensifies the extent of the steady-state concentration of two amino acids, one normal and one non-metabolizable. Another change with tissue ...
Application of glass fiber chromatography for separation of C19 steroids
(Elsevier, 1968-12)
An assay for the rates of cleavage of specific sites in DNA by restriction endonucleases: Its use to study the cleavage of phage [lambda] DNA by EcoRI and phage P22 DNA containing thymine or 5-bromouracil by HindIII
(Elsevier, 1983-03)
A method to measure the rates of cleavage of specific sites in DNAs by restriction endonucleases is described. Partial digests are prepared by incubating DNAs with limiting amounts of endonuclease. The termini generated ...