Now showing items 761-770 of 812
Analysis of Oligonucleotides by Electrospray Ionization Mass Spectrometry
(Wiley Periodicals, Inc., 2000-02)
Because of the high molecular weights and thermal lability of biomolecules such as nucleic acids and protein, they can be difficult to analyze by mass spectrometry. Such analyses require a “soft” ionization method that is ...
Selection for Catalytic Function with Nucleic Acids
(Wiley Periodicals, Inc.Cold Spring Harbor, 2000-02)
For in vitro selection of catalytic polynucleotides, each new protocol must be designed to harness the desired catalytic activity to help propel the selection process itself. This unit gives guidelines for design of in ...
Measurement of nucleoside kinases in crude tissue extracts
(Elsevier, 1983-08)
The measurements of deoxyadenosine kinase, adenosine kinase, and deoxycytidine kinase were examined in human placental cytosol to achieve a valid and reliable assay linear with time and protein. Our studies confirm the ...
Antibody-antigen complex formation with immobilized immunoglobulins
(Elsevier, 1992-08-15)
We have investigated the complex formation between an immobilized monoclonal antibody and antigens that differ in size about 50-fold. As a model system, we used an iodinated progesterone derivative and a progesterone-horseradish ...
An N-acetyl--glucosamine binding lectin from Bandeiraea Simplicifolia seeds
(Elsevier, 1976-11)
A second lectin with a high activity for nonreducing 2-acetamido-2-deoxy-[alpha]--glucopyranosyl residues has been isolated from the seed extracts of Bandeiraea simplicifolia by affinity chromatography on chitin. The lectin ...
Radiometric assay for direct quantitation of rat liver cytochrome P-450b using monoclonal antibodies
(Elsevier, 1985-08-15)
A simple and sensitive assay has been developed that is capable of detecting as little as 0.2 ng of the major isozyme of cytochrome P-450 (P-450b) isolated from the livers of phenobarbital-induced rats. This assay employs ...
Carbohydrate-binding specificity of the daffodil (Narcissus pseudonarcissus) and amaryllis (Hippeastrum hybr.) bulb lectins
(Elsevier, 1990-06)
The carbohydrate binding specificity of the daffodil (Narcissus pseudonarcissus; NPA) and amaryllis (Hippeastrum hybr.; HHA) lectins, isolated from extracts of their bulbs by affinity chromatography on immobilized mannose, ...
Protein-carbohydrate interaction : V. Further inhibition studies directed toward defining the stereochemical requirements of the reactive sites of concanavalin A
(Elsevier, 1967-07)
Inhibition studies on the concanavalin A-dextran interaction have been extended using as inhibitors the members of the maltose-, isomaltose-, and methyl [alpha]-isomaltoside series, amino and N-acetyl amino sugars, and a ...
Cumene hydroperoxide-supported denitrification of 2-nitropropane in uninduced mouse liver microsomes
(Elsevier, 1986)
1. 1. Cumene hydroperoxide supported oxidative denitrification of 2-nitropropane was investigated in uninduced mouse liver microsomes.2. 2. The cytochrome P-450 peroxygenase catalyzed reaction resulted in the production ...