Now showing items 11-20 of 31
A CE assay for the detection of agonist-stimulated adenylyl cyclase activity
(WILEY-VCH Verlag, 2007-06)
A CE assay was developed for the detection of adenylyl cyclase (AC) activity stimulated at the AC and G protein-coupled receptor (GPCR) level. In the assay, cell membranes overexpressing GPCR and/or AC were incubated with ...
Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20
(WILEY-VCH Verlag, 2006-05)
Chronic exercise training elicits adaptations in the heart that improve pump function and confer cardioprotection. To identify molecular mechanisms by which exercise training stimulates this favorable phenotype, a proteomic ...
Proteome-wide changes induced by the Hsp90 inhibitor, geldanamycin in anaplastic large cell lymphoma cells
(WILEY-VCH Verlag, 2007-08)
The molecular chaperone heat shock protein 90 (Hsp90) affects the function of many oncogenic signaling proteins including nucleophosmin-anaplastic lymphoma kinase (NPM-ALK) expressed in anaplastic large cell lymphoma (ALCL). ...
Proteomic profiling identifies breast tumor metastasis-associated factors in an isogenic model
(WILEY-VCH Verlag, 2007-01)
A combination of LC and MS was applied to an isogenic breast tumor metastasis model to identify proteins associated with a cellular phenotype. Chromatofocusing followed by nonporous-RP-HPLC/ESI-TOF MS was applied to cell ...
Measurement of dissociation rate of biomolecular complexes using CE
(WILEY-VCH Verlag, 2009-02)
Fluorescence anisotropy (FA), non-equilibrium CE of equilibrium mixtures (NECEEM) and high-speed CE were evaluated for measuring dissociation kinetics of peptide–protein binding systems. Fyn-SH3-SH2, a protein construct ...
Hypophosphorylation of the architectural chromatin protein DEK in death-receptor-induced apoptosis revealed by the isotope coded protein label proteomic platform
(WILEY-VCH Verlag, 2006-11)
During apoptosis nuclear morphology changes dramatically due to alterations of chromatin architecture and cleavage of structural nuclear proteins. To characterize early events in apoptotic nuclear dismantling we have ...
Classifications of ovarian cancer tissues by proteomic patterns
(WILEY-VCH Verlag, 2006-11)
Ovarian cancer is a morphologically and biologically heterogeneous disease. The identification of type-specific protein markers for ovarian cancer would provide the basis for more tailored treatments, as well as clues for ...
Improved Electrochemical Analysis of Neuropathy Target Esterase Activity by a Tyrosinase Carbon Paste Electrode Modified by 1-Methoxyphenazine Methosulfate
(Kluwer Academic Publishers; Springer, 2005-08)
A graphite-paste tyrosinase biosensor was improved by adding 1-methoxyphenazine methosulfate as a mediator. Mediator modification enhanced sensitivity to phenol 4-fold and long-term stability 3-fold. Phenol could be detected ...
The interaction profile of homologous recombination repair proteins RAD51C, RAD51D and XRCC2 as determined by proteomic analysis
(WILEY-VCH Verlag, 2009-08)
The RAD51 family of proteins is involved in homologous recombination (HR) DNA repair and maintaining chromosome integrity. To identify candidates that interact with HR proteins, the mouse RAD51C, RAD51D and XRCC2 proteins ...
Electrodeless direct current dielectrophoresis using reconfigurable field-shaping oil barriers
(WILEY-VCH Verlag, 2007-12)
We demonstrate dielectrophoretic (DEP) potential wells using pairs of insulating oil menisci to shape the DC electric field. These oil menisci are arranged in a configuration similar to the quadrupolar electrodes, typically ...