Now showing items 61-70 of 153
Observations consistent with autocrine stimulation of hybridoma cell growth and implications for large-scale antibody production
(Kluwer Academic Publishers; Springer Science+Business Media, 1992-04)
Existence of autocrine growth factors (aGFs) may influence the serum requirement for growth of hybridoma cells and thus significantly influence process economics. For the murine hybridoma cell line S3H5/γ2bA2, critical ...
On the Need to Develop Guidelines for Characterizing and Reporting Intrinsic Disorder in Proteins
(BIPM, SevresWiley Periodicals, Inc., 2019-03)
Since the early 2000s, numerous computational tools have been created and used to predict intrinsic disorder in proteins. At present, the output from these algorithms is difficult to interpret in the absence of standards ...
A CE assay for the detection of agonist-stimulated adenylyl cyclase activity
(WILEY-VCH Verlag, 2007-06)
A CE assay was developed for the detection of adenylyl cyclase (AC) activity stimulated at the AC and G protein-coupled receptor (GPCR) level. In the assay, cell membranes overexpressing GPCR and/or AC were incubated with ...
Label-free quantitative proteomics and SAINT analysis enable interactome mapping for the human Ser/Thr protein phosphatase 5
(WILEY-VCH Verlag, 2011-04)
Affinity purification coupled to mass spectrometry (AP-MS) represents a powerful and proven approach for the analysis of protein–protein interactions. However, the detection of true interactions for proteins that are ...
Dose‐dependent proteomic analysis of glioblastoma cancer stem cells upon treatment with γ‐secretase inhibitor
(WILEY‐VCH Verlag, 2011-12)
Notch signaling has been demonstrated to have a central role in glioblastoma (GBM) cancer stem cells (CSCs) and we have demonstrated recently that Notch pathway blockade by γ‐secretase inhibitor (GSI) depletes GBM CSCs and ...
How microbial proteomics got started
(WILEY‐VCH Verlag, 2011-08)
Publication in 1975 by Patrick O'Farrell of a procedure to separate the proteins of Escherichia coli in a two‐dimensional array on polyacrylamide gels marked the birth of the field now called proteomics. Although O'Farrell's ...
Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20
(WILEY-VCH Verlag, 2006-05)
Chronic exercise training elicits adaptations in the heart that improve pump function and confer cardioprotection. To identify molecular mechanisms by which exercise training stimulates this favorable phenotype, a proteomic ...
Proteome-wide changes induced by the Hsp90 inhibitor, geldanamycin in anaplastic large cell lymphoma cells
(WILEY-VCH Verlag, 2007-08)
The molecular chaperone heat shock protein 90 (Hsp90) affects the function of many oncogenic signaling proteins including nucleophosmin-anaplastic lymphoma kinase (NPM-ALK) expressed in anaplastic large cell lymphoma (ALCL). ...