Now showing items 361-370 of 416
Enhanced sensitivity electrochemical assay of low-molecular-weight heparins using rotating polyion-sensitive membrane electrodes
(Springer-Verlag, 2003-11)
Use of a novel rotating polycation-sensitive polymer membrane electrode yields sensors that can serve as simple potentiometric titration endpoint detectors for the determination of three FDA approved low-molecular-weight ...
Synthesis of Protected 2′‐Deoxy‐2′‐fluoro‐β‐D‐arabinonucleosides
(Wiley Periodicals, Inc.International Society for Nucleosides, Nucleotides, and Nucleic Acids, 2002-09)
This unit describes in detail the preparation of protected 2’‐deoxy‐2’‐fluoroarabinonucleosides. These building blocks are required for the synthesis of 2’‐deoxy‐2’‐fluoroarabinonucleic acid (2’F‐ANA), an oligonucleotide ...
The role of DNA synthesis inhibition in the cytotoxicity of 2′,2′-difluoro-2′-deoxycytidine
(Springer-Verlag, 2003-10)
Cytotoxicity from the anticancer drug 2′,2′-difluoro-2′-deoxycytidine (dFdCyd) has been correlated with its incorporation into DNA. However, cytotoxicity may also result from inhibition of DNA synthesis, due to either (1) ...
Probing RNA Folding Pathways by RNA Fingerprinting
(John Wiley & Sons, 2000-10)
This unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or ...
Analyzing Radioligand Binding Data
(Raven Press,Wiley Periodicals, Inc., 2000-09)
A radioligand is a radioactively labeled drug that can associate with a receptor, transporter, enzyme, or any protein of interest. Measuring the rate and extent of binding provides information on the number of binding ...
Terephthalic acid synthesis at higher concentrations in high-temperature liquid water. 2. Eliminating undesired byproducts
(Wiley Subscription Services, Inc., A Wiley Company, 2009-06)
We synthesized terephthalic acid (TPA) from p -xylene at an initial concentration above its solubility limit in high-temperature liquid water (HTW). The nominal p -xylene loading at the reaction conditions was 0.4 mol L ...
Cartridge Methods for Oligonucleotide Purification
(Wiley Periodicals, Inc., 2000-07)
Protocols are given for purification of oligonucleotides by dimethoxytrityl‐sensitive and affinity desalting methods. The protocols are applicable for many of the convenient disposable products available for rapid ...
Micropatterned Fiber Scaffolds for Spatially Controlled Cell Adhesion
(WILEY-VCH Verlag, 2009-10-01)
Because the local microstructure plays a pivotal role for many biological functions, a wide range of methods have been developed to design precisely engineered substrates for both fundamental biological studies and ...
A proteomic analysis of Psychrobacter articus 273-4 adaptation to low temperature and salinity using a 2-D liquid mapping approach
(WILEY-VCH Verlag, 2007-02)
Psychrobacter 273-4 was isolated from a 20 000–40 000–year-old Siberian permafrost core, which is characterized by low temperature, low water activity, and high salinity. To explore how 273-4 survives in the permafrost ...
Chemistry of CpG DNA
(Wiley Periodicals, Inc., 2003-03)
The vertebrate immune system can recognize specific pathogen‐associated molecular patterns in invading microorganisms, including the unmethylated CpG dinucleotide. This unit discusses the receptors that recognize CpG motifs ...