Now showing items 1391-1400 of 1401
Affinity isolation of RNA polymerase II on amanitin-Sepharose
(Elsevier, 1984-02-29)
We report here the first case of an affinity isolation of eukaryotic RNA polymerase II. The procedure employs an affinity matrix composed of [alpha]-amanitin coupled to Sepharose 4B via a ten atom spacer. RNA polymerase ...
Fluid shear stress stimulates breast cancer cells to display invasive and chemoresistant phenotypes while upregulating PLAU in a 3D bioreactor
(Wiley Periodicals, Inc., 2019-11)
Breast cancer cells experience a range of shear stresses in the tumor microenvironment (TME). However most current in vitro three‐dimensional (3D) models fail to systematically probe the effects of this biophysical stimuli ...
Microporous scaffolds loaded with immunomodulatory lentivirus to study the contribution of immune cell populations to tumor cell recruitment in vivo
(Wiley Periodicals, Inc., 2020-01)
Metastases are preceded by stochastic formation of a hospitable microenvironment known as the premetastatic niche, which has been difficult to study. Herein, we employ implantable polycaprolactone scaffolds as an engineered ...
Understanding Human Lung Development through In Vitro Model Systems
(Wiley Periodicals, Inc., 2020-06)
An abundance of information about lung development in animal models exists; however, comparatively little is known about lung development in humans. Recent advances using primary human lung tissue combined with the use of ...
The Anti‐G0 Manifesto: Should a problematic construct (G0) with no biological reality be removed from the cell cycle? Yes!
(Wiley Periodicals, Inc., 2021-03)
It is widely accepted that there exists a “resting” or “quiescent” state where a growing cell leaves the cell cycle to enter what is often called the “G0‐phase.” I propose that there is no biological reality to the “G0‐phase.” ...
CFTR Does Not Alter Acidification of L Cell Endosomes
(Elsevier, 1994-11-30)
Endosomes from L cells, transduced with the CFTR gene, and the parental line, which does not express detectable levels of CFTR, were loaded with FITC-dextran, isolated and the initial rates of acidification, steady-state ...
Disparate gene expression of chemotactic cytokines by human mononuclear phagocytes
(Elsevier, 1990-01-30)
Chemotactic cytokines are becoming increasingly recognized as important participants in the coordinate recruitment of specific inflammatory cells. In this manuscript we present data demonstrating that LPS challenged human ...
Large unit red cell cryopreservation with hydroxyethyl starch
(Elsevier, 1976-10)
Full units of red blood cells frozen with 14% hydroxyethyl starch (HES) yield cell recoveries near 97% and saline stabilities greater than 80%. Potassium leaves the cells during the freeze-thaw cycle and increases the ...
Soil respiration in northern forests exposed to elevated atmospheric carbon dioxide and ozone
(Springer-Verlag, 2006-02-18)
The aspen free-air CO 2 and O 3 enrichment (FACTS II–FACE) study in Rhinelander, Wisconsin, USA, is designed to understand the mechanisms by which young northern deciduous forest ecosystems respond to elevated atmospheric ...
Microclimatic changes and the indirect loss of ant diversity in a tropical agroecosystem
(Springer-Verlag, 1996-11)
Recent changes in the coffee agroecosystem of Costa Rica were used to study the mechanism of biodiversity loss in transforming agroecosystems, focusing on the ground-foraging ant community. Coffee farms are being transformed ...