Now showing items 91-100 of 143
Oxygen requirement for prostaglandin biosynthesis
(Elsevier, 1978-08)
The formation of prostaglandins by vesicular gland cyclooxygenase can be regulated by O2 concentrations below 30 [mu] (20 mm Hg) with an apparent Km value of about 5 [mu]M. This result suggests that most mammalian tissues ...
Activity of prostaglandin E, F, A and B on sphincter, dilator and ciliary muscle preparations of the cat eye
(Elsevier, 1975-02)
Both sphincter and dilator muscle preparations of the cat iris contract to prostaglandins; F2[alpha] and E2 are the most potent and A1 and B1 the least. Ciliary muscle strips relax to PG's provided that the strips are ...
Instrumental improvements for rapid, detailed kinetic studies of oxygenase activity
(Elsevier, 1979-07-15)
The characteristics of an electronic differentiator that facilitates continuous monitoring of changes in the oxygen concentration measured by a polarographic oxygen electrode are described. In addition, some properties of ...
A specific, sensitive method for the determination of hyaluronate
(Elsevier, 1979-07-15)
A sensitive and specific assay for hyaluronate was devised. Hyaluronate contained in biological mixtures was digested with a commercially available microbial hyaluronate lyase. The [beta]-[Delta]4,5-eneglucopyranuronic ...
Binding and circular dichroism data on bilirubin-albumin in the presence of oleate and salicylate
(Elsevier, 1970-09)
The binding of equimolar amounts of bilirubin to human and bovine serum albumin in 0.1 phosphate buffer, pH 7.4, in the presence and absence of various concentrations of oleate or salicylate was studied by the use of an ...
Formation of palmityl-[3'-32P]coenzyme a from [[gamma]-32P]ATP in mitochondrial extracts of guinea pig liver
(Elsevier, 1977-01-15)
Investigations of the incorporation of 32P into acyl-coenzyme A (CoA) in incubation mixtures containing a soluble protein preparation derived from mitochondria, [[gamma]-32P]ATP, and palmityl-CoA have led to the discovery ...
Effect of a diabetogeic factor from bovine adenohypophysis on dog serum nefa levels
(Elsevier, 1972-10)
1. 1. Fasted dog serum non-esterified fatty acid (NEFA) levels were elevated 10 and 34 hours after administration of a diabetogenic protein (BDF) isolated from bovine adenohypophysis.2. 2. The fasting serum NEFA were ...
Aldonate coupling, a simple procedure for the preparation of carbohydrate-protein conjugates for studies of carbohydrate-binding proteins
(Elsevier, 1976-08)
Aldonate coupling a new, high-yield method for conjugation of reducing oligosaccharides to proteins is described. It involves oxidation of the oligosaccharides to their corresponding aldonic acids followed by coupling to ...
Comparative study of two highly purified forms of liver microsomal cytochrome P-450: Circular dichroism and other properties
(Elsevier, 1979-06)
The two major forms of rabbit liver microsomal cytochrome P-450, P-450LM2 and P-450LM4, which were previously shown to differ in their absorption spectra, electrophoretic and immunochemical properties, and substrate ...