Now showing items 111-120 of 143
A specific, non-chromatographic radioimmunoassay for human plasma cortisol
(Elsevier, 1975-11)
A radioimmunoassay technique has been developed for the measurement of cortisol in a single methylene chloride extract of human plasma without chromatography. The antiserum, obtained by immunizing rabbits with cortisol-3 ...
A rapid sensitive assay for phosphatidate phosphohydrolase
(Elsevier, 1978-06-15)
For a purified preparation of the soluble form of phosphatidate phosphohydrolase (EC 3.1.3.4) from guinea pig cerebral cortex, 1-O-alkyl-rac-glycerol 3-phosphate was found to be accepted as a substrate. This substrate ...
Sensitive radioimmunoassay for digoxin in plasma and urine
(Elsevier, 1977-06)
A reproducible and sensitive radioimmunoassay for digoxin in either serum, plasma or urine is described. Using 0.5 ml of serum or plasma, the assay sensitivity is 0.05 ng of digoxin/ml. The antiserum and tracer solutions ...
Plasma prednisolone concentrations: Comparison of radioimmunoassay and competitive protein binding assay
(Elsevier, 1977-05)
A comparison was made between plasma concentrations of prednisolone measured by both competitive protein binding radioassay (CPB) and radioimmunoassay (RIA) and, with each assay, using a calibration curve generated from ...
Fluorographic detection of radioactivity in polyacrylamide gols with the water-soluble fluor, sodium salicylate
(Elsevier, 1979-09-15)
Sodium salicylate has been found to serve as a satisfactory fluor for enhanced detection of radioactivity in polyacrylamide gels. The compound is inexpensive, water soluble, penetrates rapidly into gels, and gives linearity ...
Tumor localizing agents. IX. Radioiodinated cholesterol
(Elsevier, 1970)
19-Iodocholesterol-125I was synthesized for study as a possible agent for photoscanning the adrenal gland and associated tumors. In contrast to previous radioiodinated steroids, it was found to be much less prone to rapid ...
Prostaglandin biosynthesis can be triggered by lipid peroxides
(Elsevier, 1979-04-01)
Studies of ferriheme cyclooxygenase, using two different assay systems, show that a variety of peroxides can trigger a rapid acceleration of cyclooxygenase activity to produce prostaglandins. Lipid hydroperoxides formed ...
Anions and the contraction of glycerol-extracted muscle fibers
(Elsevier, 1970-01)
Glycerinated rabbit psoas fibers 5-19 days old were immersed in contracting solutions with and without varying concentrations of different sodium salts. Raising the pH of the solution in small steps identified a threshold ...
Enzymatic determination of cardiac glycosides
(Elsevier, 1971-08)
A specific and highly sensitive enzymatic method for the determination of cardiac glycosides has been developed. The method is based on the inhibition of the Na,K-ATPase in a microsomal preparation from beef cerebral cortex. ...