Now showing items 121-130 of 143
Properties of spinach ferredoxin in anaerobic urea solution: A comparison with the native protein
(Elsevier, 1970-12)
The preparation of spinach ferredoxin by a modification of the method of Keresztes-Nagy and Margoliash which is particularly convenient for large-scale operation is described.The optical, CD, and EPR spectra of this protein ...
Hydrogen peroxide formation and stoichiometry of hydroxylation reactions catalyzed by highly purified liver microsomal cytochrome P-450
(Elsevier, 1977-04-30)
The stoichiometry of hydroxylation reactions catalyzed by cytochrome P-450 was studied in a reconstituted enzyme system containing the highly purified cytochrome from phenobarbital-induced rabbit liver microsomes. Hydrogen ...
A new class of model glycolipids: Synthesis, characterization, and interaction with lectins
(Elsevier, 1979-06)
A method is described for preparing model glycolipids by linking aldobionic acids to an alkylamine through an amide bond. These compounds may be rapidly prepared in large quantities. The glycolipids precipitate specifically ...
A support for affinity chromatography that covalently binds amino groups via a cleavable connector arm
(Elsevier, 1979-03)
The preparation of a new succinimidyl ester agarose derivative (SEPE-Agarose) is described. This agarose derivative can be used for covalently linking proteins and other ligands containing amino groups to agarose via phenyl ...
Cadmium and arsenite binding by N-dihydrolipoyl-aminoethoxydextran: A model study of enzyme dithiol criteria
(Elsevier, 1973)
We have observed the interaction of Cd2+, Zn2+, and AsO22- with a dithiol-substituted polymer (N-dihydrolipoyl aminoethoxy dextran). Cadmium binding results in an intense uv difference spectrum ([lambda]max = 240 nm). ...
Epimerization of trans-4-hydroxy--proline to cis-4-hydroxy--proline during acid hydrolysis of collagen
(Elsevier, 1972-10)
1. 1. trans-4-Hydroxy--proline is epimerized to cis-4-hydroxy--proline under the conditions generally used for the hydrolysis of proteins for analyses of amino acids.2. 2. The cis epimer elutes from a column of UR-30 resin ...
Immunochemical studies on methyl [alpha]- and methyl [beta]-glucosaminide-azoprotein conjugates
(Elsevier, 1976-09)
Conjugates consisting of methyl 2(p-azobenzamido)2-deoxy-[alpha]--glucopyranoside and methyl 2(p-azobenzamido)-2-[beta]--glucopyranoside coupled to bovine serum albumin were used as synthetic antigens. Rabbits immunized ...
Correlation between serum enzymes, isozyme patterns and histologically detectable organ damage
(Elsevier, 1971)
Serum enzyme levels and isozyme profile were utilized as a measure of hepatotoxic response to carbon tetrachloride, mercuric chloride, diethanolamine and thioacetamide. The sensitivity of these measurements was compared ...