Now showing items 251-260 of 336
Characterization of pro-opiomelanocortin processing in heterologous neuronal cells that express PC2 mRNA
(Elsevier, 1993-05)
We have investigated processing of monkey pro-opiomelanocortin (POMC) following transfection into heterologous neuronal Neuro 2A (N2A) cells. In several separately transfected stable cell lines (termed N2A/POMC2-like; N = ...
Conformational studies of hairpin sequences from the ColE1 cruciform
(Elsevier, 1993)
Inverted repeat sequences derived from the ColE1 cruciform were investigated by nuclear magnetic resonance (NMR) and UV spectroscopy. It was shown that 15 different sequences exist as stable hairpin structures over a range ...
Wortmannin Blocks Goldfish Retinal Phosphatidylinositol 3-Kinase and Neurite Outgrowth
(Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media, 1997-04)
The goldfish retina has been used extensively for the study of nerve regeneration. A role for phosphatidylinositol 3-kinase (PI3K) in neurite outgrowth from goldfish retinal explants has been examined by means of wortmannin ...
Systematic approach to the development of plasma amino acid analysis by high-performance liquid chromatography with ultraviolet detection with precolumn derivatization using phenyl isothiocyanate
(Elsevier, 1993-11-17)
A reversed-phase liquid chromatographic method for the separation of 26 phenylthiocarbamyl derivatives of amino acids in human plasma in ca. 35 min. is described. The method used a C18 column (150 x 4.6 mm I.D., 3 [mu]m) ...
Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli
(Elsevier, 1990-12)
DNA polymerase from Thermus aquaticus has become a common reagent in molecular biology because of its utility in DNA amplification and DNA sequencing protocols. A simplified method is described here for isolating the ...
Cloning and characterization of a pharmacologically distinct A1 adenosine receptor from guinea pig brain
(Elsevier, 1994-10)
Three full-length cDNA clones encoding the guinea pig A1 adenosine receptor have been isolated by polymerase chain reaction (PCR) and low-stringency hybridization screening of a guinea pig brain cDNA library. These three ...
Hydrogen Peroxide-Dependent Formation and Bleaching of the Higher Oxidation States of Bovine Erythrocyte Green Hemeprotein
(Elsevier, 1993-02-15)
The ferric and ferrous forms of bovine erythrocyte green hemeprotein react with hydroperoxides to form higher oxidation state intermediates with absorbance maxima in the Soret region at 426 and 422 nm, respectively. In the ...
A potential animal model for studying CF heterozygote advantage: Genetic variation in theophylline-inducible colonic chloride currents among inbred strains of mice
(Elsevier, 1992-02)
We have used Ussing chambers to measure chloride secretion by colonic segments (mucosa, muscularis, and serosa) from various inbred strains of mice. We found lower theophylline-induced Cl- secretion in the DBA/2J than in ...
Defined Analyte-Enzyme Conjugates as Signal Generators in Immunoassays
(Elsevier, 1993-04)
We investigated the synthesis of progesterone-horseradish peroxidase (P-HRP) conjugates and products purified by affinity chromatography. The obtained preparations were characterized with an immobilized monoclonal antibody ...