Now showing items 51-60 of 160
Syntheses of Specifically 15N‐Labeled Adenosine and Guanosine
(Wiley Periodicals, Inc., 2002-09)
This unit describes the specific incorporation of 15N into the N7 and amino positions of adenosine, and conversion of the adenosine to guanosine labeled at the N1, N7, and amino positions. Two variations of the procedures ...
Chemical Synthesis of RNA Sequences with 2′‐O‐[(Triisopropylsilyl)oxy]methyl‐protected Ribonucleoside Phosphoramidites
(Oxford University PressWiley Periodicals, Inc., 2001-12)
The [(triisopropylsilyl)oxy]methyl (TOM) group is a useful protecting group for the 2′‐OH of ribonucleosides to be used for oligoribonucleotide synthesis by the phosphoramidite method. It is completely stable to all reaction ...
Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation (BiFC) Analysis
(Wiley Periodicals, Inc., 2005-08)
Protein interactions integrate stimuli from different signaling pathways and developmental programs. Bimolecular fluorescence complementation (BiFC) analysis has been developed for visualization of protein interactions in ...
IUPAC‐IUB Joint Commission on Biochemical Nomenclature Abbreviations and Symbols for the Description of Conformations of Polynucleotide Chains
(Academic PressWiley Periodicals, Inc., 2000-02)
This appendix presents nomenclature rules that are as close as possible to the originally published version and are reproduced here with permission from the European Journal of Biochemistry. A web‐based version prepared ...
In Vitro Selection Using Modified or Unnatural Nucleotides
(Wiley Periodicals, Inc.University of Texas, 2001-12)
The use of modified nucleotides in an RNA or DNA pool to be used for in vitro selection offers many potential advantages, such as the increased stability of the selected nucleic acid against nuclease degradation. This unit ...
Overview of Purification and Analysis of Synthetic Nucleic Acids
(Applied BiosystemsWiley Periodicals, Inc., 2000-07)
Synthetic nucleic acids are produced routinely for a wide variety of applications, including biological and chemical research, and diagnostic or therapeutic applications. To ensure an adequate level of quality and purity, ...
Synthesis of 5′‐O‐Phosphoramidites with a Photolabile 3′‐O‐Protecting Group
(Wiley Periodicals, Inc., 2004-06)
This unit describes the chemical synthesis of phosphoramidite building blocks that carry a protecting group at the 3′ position. These inversely oriented synthons expose a 2‐(2‐nitrophenyl)propoxycarbonyl (NPPOC) group as ...
Oligodeoxyribonucleotide Analogs Functionalized with Phosphonoacetate and Thiophosphonoacetate Diesters
(Humana PressWiley Periodicals, Inc., 2004-09)
Oligodeoxyribonucleotides with phosphonoacetate or thiophosphonoacetate internucleotide linkages can be made in high yield by solid‐phase synthesis and possess many advantages. They are highly stable to nucleases, ...