Now showing items 21-30 of 68
Loss of antibody productivity is highly reproducible in multiple hybridoma subclones
(Wiley Subscription Services, Inc., A Wiley Company, 1993-06-20)
An immunoglobulin G (IgG 2b ) producing hybridoma cell line (S3H5/Γ2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum-free medium. Following adaptation ...
Lactate Oxidation for the Detection of Mitochondrial Dysfunction in Human Skin Fibroblasts
(Elsevier, 1993-05-01)
To screen fibroblasts for defects in lactate/pyruvate oxidation, cells were grown to confluence in 25-cm2 flasks, rinsed, and incubated in glucose-free media containing 25 [mu]M L-lactate and 0.1 [mu]Ci [D,L-1-14C]lactate. ...
Infectivity of a glucan synthesis-defective mutant of Streptococcus gordonii (Challis) in a rat endocarditis model
(Elsevier, 1993-09-15)
Streptococcus gordonii, a member of the human indigenous oral microflora, colonizes smooth tooth surfaces and contributes to dental plaque formation. Although it is not recognized as being a cariogenic pathogen, it may ...
Synthesis of 1-(2-aminopropyl)benzimidazoles, structurally related to the TIBO derivative R82150, with activity against human immunodeficiency virus.
(Elsevier, 1993-04)
A number of 1-(2-aminopropyl)-2-mercaptobenzimidazoles related to the TIBO derivatives R82150 have been prepared and tested for their activity against human immunodeficiency virus type 1 (HIV-1). These compounds were all ...
S18.20 Preparation of two kinds of sialic acid specific monomeric monovalent lectin derivatives and their application to the study of cell surface glycoconjugates by flow cytometry
(Kluwer Academic Publishers; Chapman & Hall ; Springer Science+Business Media, 1993-08)
Progesterone modulation of gonadotropin secretion by dispersed rat pituitary cells in culture. IV. Follicle-stimulating hormone synthesis and release
(Elsevier, 1993-02)
Estradiol-treated, rat pituitary cells were studied to examine the effects of progesterone (P) on follicle-stimulating hormone (FSH) synthesis and secretion. Progesterone was administered prior to or concurrent with 3 h ...
Rapid analysis of leukocyte-endothelial adhesion
(Elsevier, 1993-02-26)
Neutrophil adherence to endothelial monolayers in multi-well plates can be rapidly quantitated, using a fluorescent plate reader. The number of adherent neutrophils which have been labeled with biscarboxyethyl carboxyfluorescein ...
The effects of transforming growth factor alpha and somatostatin on regenerating hepatocytes in the rat
(Elsevier, 1993-03-05)
Transforming growth factor alpha (TGF[alpha]) stimulates DNA synthesis in adult rat hepatocytes, and plays a physiological role after partial hepatectomy by an autocrine mechanism. Somatostatin (SS-14) is a potent inhibitor ...
Potentiometric detection in ion chromatography using multi-ionophore membrane electrodes
(Elsevier, 1993-10-01)
The incorporation of ionophores selective for ammonium, potassium, sodium and calcium ions in an appropriate proportion into a plasticized poly(vinyl chloride) membrane provides a potentiometric membrane sensor with similar ...